池蝶蚌mtDNA COⅠ基因序列分析  

Sequence Analysis of Mitochondrial COⅠ of Hyriopsis schlegeli

作　　者：王军花[1] 曾柳根[1] 林巧惠[1] 盛军庆[1] 徐毛喜 龚贵如[1] 洪一江[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,江西南昌330031 [2]江西抚州市洪门水库开发公司,江西抚州344712

出　　处：《水生态学杂志》2010年第5期21-26,共6页Journal of Hydroecology

基　　金：农业部948项目(2004-T9);国家发改办([2005]1899)资助

摘　　要：以三角帆蚌(Hyriopsis cumingii)线粒体DNA设计特异性引物,对池蝶蚌(H.schlegelii)的线粒体COⅠ基因进行PCR扩增,以期了解池蝶蚌的选育效果。结果表明:在10个个体中均得到序列一致的COⅠ序列,长度为1542bp;A、T、G、C含量分别为18.1%(280个)、41.8%(644个)、25.4%(392个)和14.7%(226个),A+T含量(59.9%)明显高于G+C含量(40.1%),与其他无脊柱动物相同基因片段碱基含量相似。该基因中密码子T(U)的使用频率很高,第1位核苷酸中T和G的含量较为一致分别为32.0%和30.8%;密码子第2位上碱基T的使用比率高达42.7%,碱基G的使用比率低至17.2%;密码子第3位上碱基T的使用比率高达50.7%,而碱基C的使用比率仅为6.6%。BLAST结果显示池蝶蚌与其他淡水贝类的COⅠ基因具有较高的同源性,其中与三角帆蚌的相似性为95%,MP法构建的分子系统进化树表明:池蝶蚌COⅠ基因与三角帆蚌COⅠ基因亲缘关系最近。以上结果表明:池蝶蚌经过人工选育后已初步成为一个纯系,遗传性状趋于稳定。With the expectation to investigate the breeding effect of Hyriopsis schlegeli, mitochondrial DNA COⅠ gene was amplified by PCR and sequenced by two primers which were designed according to the complete mtDNA of Hyriopsis cumingi. The results showed that there was no difference in sequences among the ten samples. The number of its whole gene sequence was 1542 bp in length which had 18.1% （280） ,41. g % （644） ,25.4 % （392）, 14.7% （226） of A, T, G, and C, respectively, A ＋ T（59.9% ） 〉 G ＋ C（40.1% ）, similar to the sequences in other invertebrate animals. It had high content of T in cidons, the first positions of codons had 32.0 % content of T and 32.8 % content of G; the second positions of the codons in the gene have high content of T（42.7） but low content of G（ 17.2% ） ; 50.7 % content of T and 6.6 % content of C were found in the third positions. The COⅠgene of the H. Schelegii was proved to have good homology with other species of freshwater mussel recorded in the GenBank, the sequence similarity between H. Schelegii and 11. cumingi, was 95%. The result of Phylogenetic tree which was constructed by Maximum Parsimony method indicated that there was the closest phylogenetic relationship between H. Schelegii and H. cumingi. It indicated that the genetic characteristic of the H. schlegeli tends to be stable and become pure strain after 3 generation artificial selected.

关 键 词：池蝶蚌 线粒体 COⅠ基因 人工选育 序列分析 

分 类 号：Q523[生物学—生物化学]