灿烂弧菌和创伤弧菌的不同保存方法比较  

Comparison of Different Preservation Methods for Vibrio splendidus and Vibrio vulnificus

作　　者：闫茂仓[1] 陈少波[1] 单乐州[1] 王雪鹏[2] 马爱敏[1] 谢起浪[1] 

机构地区：[1]浙江省海洋水产养殖研究所浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005 [2]山东农业大学动物科技学院,山东泰安271018

出　　处：《水生态学杂志》2010年第5期136-140,共5页Journal of Hydroecology

基　　金：浙江省科技厅科研院所专项公益技术攻关项目(2007F10009);浙江省科技厅科研院所青年人才计划项目(2006R20001);温州市科技兴海项目(S20070051)联合资助

摘　　要：比较了琼脂斜面法、穿刺石蜡法和甘油冷冻法保存灿烂弧菌(Vibrio splendidus)和创伤弧菌(Vibrio vulnifi-cus)的效果。结果表明,甘油冷冻法和穿刺石蜡法的保存期显著比琼脂斜面法长,保存12月后,各保存方法细菌的毒力都有不同程度的下降,甘油冷冻法显著好于其它2种保存方法。Three preservation methods （serially passage on bevel method, puncture-paraffin method, glycerol crypreservation method） for same strain of Vibrio splendidus and Vibrio vulnificus were compared in this paper. The result showed that the preserved stage of glycerol cry-preservation method and puncture-paraffin method was longer than anther method; the virulence of Listonella anguillarum preserving twelve months decreased in different degree for three preservation methods, glycerol cry-preservation method was better than the others.

关 键 词：灿烂弧菌 创伤弧菌 穿刺石蜡法 甘油冷冻法 

分 类 号：S852.6[农业科学—基础兽医学]