褐飞虱长短翅型成虫不同组织内Wolbachia的密度  

Infection density of Wolbachia in different tissues of macropters and brachypters adults of Nilaparvata lugens (Stl)

作　　者：张慧[1] 张开军[1] 洪晓月[1] 

机构地区：[1]南京农业大学昆虫学系,江苏南京210095

出　　处：《南京农业大学学报》2010年第5期35-39,共5页Journal of Nanjing Agricultural University

基　　金：国家973计划项目(2006CB102001);公益性行业(农业)科技专项项目(nyhyzx-200803003)

摘　　要：以褐飞虱海南三亚种群为试验材料,运用实时荧光定量PCR方法对感染Wolbachia品系的长翅雌虫、长翅雄虫、短翅雌虫和短翅雄虫体内Wolbachia的密度进行测定。研究结果表明:雌虫中Wolbachia的密度一般都高于雄虫,短翅雌虫中Wolbachia的密度要显著高于长翅型雌虫。进一步对虫体头部、胸部、腹部和足4个组织的Wolbachia含量进行了测定,研究其在寄主各个组织中含量分布的差异。4个组织中,由于褐飞虱的生殖组织在腹部,而Wolbachia主要集中于寄主的生殖细胞中,所以腹部的Wolbachia含量最高;在非生殖组织中,同样存在Wolbachia,胸部含量比头部和足的含量都要高。The Hainan population of Nilaparvata lugens （ Stl） was used as material and the density of Wolbachia in macropters and brachypters of N. lugens was measured using real-time fluorescent quantitative PCR method. It was shown that the Wolbachia load was higher in females than in males and the macropterous females harbored more Wolbachia than brachypterous ones. The N. lugens was dissected into four parts （ head,thorax,abdomen and six legs） and the infection amount of Wolbachia in each part was detected. The highest infection load in the abdomen of adults could be explained by that Wolbachia often concentrated in the gonads which are in the abdomen. In the somatic,we also found the wide distribution of Wolbachia. The infection density of Wolbachia in the baenosome was higher than in the cephalosome and legs.

关 键 词：WOLBACHIA 褐飞虱 翅型 实时荧光定量PCR 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]