水分胁迫下玉米叶片中一氧化氮与钙和钙调素的相互作用  

Interplay between nitric oxide and calcium/calmodulin in maize leaves (Zea mays L.) exposed to water stress

作　　者：桑建荣[1] 蒋明义[2] 陈永昌[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]南京农业大学生命科学学院,江苏南京210095

出　　处：《南京农业大学学报》2010年第5期111-115,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30571122)

摘　　要：以玉米(Zea mays L.)为材料,研究水分胁迫下玉米叶片中NO与Ca2+和钙调素(CaM)含量之间的关系。结果显示:Ca2+鳌合剂、Ca2+通道阻塞剂和CaM拮抗剂预处理均抑制了水分胁迫诱导的NO产生和一氧化氮合酶(nitric ox-idesynthase,NOS)活性的提高,同时也能抑制水分胁迫诱导的叶片线粒体NO产生,而对叶绿体NO产生并没有影响;NO清除剂羟基2苯基4,4,5,5四咪唑1羟基3氧化物(2-4-carboxypheny l-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide,c-PTIO)能抑制水分胁迫条件下Ca2+和CaM的产生以及CaM1基因表达,但对水分胁迫最初诱导的玉米叶片叶肉细胞原生质体Ca2+和玉米叶片中CaM1基因表达以及CaM含量增加没有影响。上述结果表明:水分胁迫下NO和Ca2+/CaM之间存在相互作用。In this study,the relationship between calcium （ Ca^2＋ ） /calmodulin （ CaM） levels and NO production was investigated in leaves of maize （ Zea mays L. ） exposed to water stress. Pretreatments with Ca^2＋ inhibitors or CaM antagonists inhibited water stress-induced NO production and the activity of NOS in cytosolic and microsomal fractions of maize leaves. Interestingly, blockade of Ca^2＋ /CaM with Ca^2＋ inhibitors or CaM antagonists could only reduce NO level in mitochondria but not in chloroplast. Exogenous NO increased levels of Ca^2＋ /CaM,expression of the camodulin 1 （ CaM1） and content of CaM under water stress. However,the NO scavenger,2-4-carboxypheny 1-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide （ c-PTIO） ,did not affect water stress-induced the initial increase of Ca^2＋ in the protoplasts of mesophyll cells. There existed an interplay between NO and Ca^2＋ / CaM in maize leaves exposed to water stress.

关 键 词：CA^2+/CAM NO NOS 水分胁迫 玉米 

分 类 号：Q945.78[生物学—植物学]