贵州苗药胡三贴质量标准的研究  

Quality Standard for Three Patches of Hushi

作　　者：靳凤云[1] 马克芹[1] 胡世玺 高源[1] 孙宜春[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]贵州贵定胡三贴药械有限公司贵州,贵定5513003

出　　处：《中国实验方剂学杂志》2010年第14期44-47,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省优秀科技教育人才省长专项资金项目:黔省专合字(2008)65

摘　　要：目的:建立胡三贴的质量标准。方法:采用薄层色谱法对方中铁筷子、延胡索和威灵仙进行鉴别;采用高效液相色谱法测定了方中君药淫羊藿中淫羊藿苷的含量。结果:薄层色谱斑点清晰,分离效果良好,专属性强,阴性对照无干扰;淫羊藿苷在0.195 2～1.659 2μg呈现良好的线性关系(r=0.999 8);平均加样回收率为96.29%,RSD为1.05%(n=9)。结论:本法快速、简便、重复性好、回收率高,结果准确可靠,可用于胡三贴的质量控制。Objective：To establish a quality standard for Three Patches of Hushi.Method：Helleborus thi-betanus,Rhizoma Corydalis,Clematis chinensis Osbeck were identified by TLC,and the content of icariin was de-termined by HPLC.Result：Helleborus thibetanus,Rhizoma Corydalis,Clematis chinensis Osbeck could be identi-fied by TLC,icariin was linear in the range of 0.195 2-1.659 2 μg,r = 0.999 8.The average recovery was 96.29%,RSD 1.05%（n = 9）.Conclusion：This method was accurate and can be used for the quality control of Three Patches of Hushi.

关 键 词：胡三贴 薄层色谱法 高效液相色谱法 淫羊藿苷 

分 类 号：R284.1[医药卫生—中药学]