不同产地野菊花中蒙花苷含量与红外光谱的对比  

Study on Content of Buddleoside from Flos Chrysanthemi Indici and Their Identification by Infrared

作　　者：吴明侠[1] 王晶娟[2] 张贵君[2] 孙素琴[3] 

机构地区：[1]河南中医学院,郑州450008 [2]北京中医药大学,北京100102 [3]清华大学化学系,北京100084

出　　处：《中国实验方剂学杂志》2010年第14期54-57,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南中医学院博士科研基金(BSJJ2009-11)

摘　　要：目的:建立测定野菊花中蒙花苷含量的方法,并且与其红外光谱进行对比研究。方法:以不同产地的供试品为载体,采用HPLC法测定含量,Diamonsil C18色谱柱(4.6mm×250 mm,5μm);流动相A相为0.5%磷酸水溶液,B相为乙腈,进行梯度洗脱,在0～10 min,B相从10%线性改变至20%,10～30 min,B相从20%线性改变至30%;30～40 min,B相从30%线性改变至40%;流速1.0 mL.min-1;检测波长326 nm;柱温35℃。IR光谱采用傅里叶变换红外光谱技术,获得野菊花的红外谱,二阶导数谱和二维相关红外谱。结果:蒙花苷的线性范围为0.262～5.24μg(r=0.999 91),回收率为98.75%,RSD为1.02%。不同产地野菊花由于其中所含蒙花苷含量的差异,在IR二阶导数谱上1 700～1 400 cm-1及二维红外光谱在1 800～800 cm-1波段均有明显区别。结论:建立的HPLC方法测定蒙花苷含量准确、快速、重复性好,适合于测定野菊花中黄酮类成分的含量。野菊花中蒙花苷含量的不同在IR光谱上可以看出差异,可以作为鉴别其质量的参考指标之一。Objective：To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method：Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18（4. 6 mm × 250 mm,5 μm） column gradient eluted with a mobile phase of 0. 5% H3PO4（A）-acetonitrile（B）,B changed from 10% to 20% in the first 10 min,in the next 20 min,when B changed from 20% to 30% ,and in the last 10 min,B changed to 40% . The detection wavelength was set at 326 nm and column temperature was set at 35 ℃ . The FTIR spectrums,the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result：Buddleoside was linear within the range of 0. 262-5. 24 μg （r = 0. 999 91）. The average recovery was 98. 75% ,with RSD 1. 02% （n = 5）. There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700-1 400 cm -1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800-800 cm -1. Conclusion：The method was accurate,repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthe- mi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.

关 键 词：高效液相色谱法 傅里叶变换红外光谱法 野菊花 蒙花苷 

分 类 号：R284.1[医药卫生—中药学]