内标多控法测定枳实、枳壳、青皮和陈皮中黄酮类成分含量  

Determination of Flavone Ingredients in Citrus Aurantium,Bitter Orange Immature,Walnut Peel and Tangerine Peel By Internal Standard Multi-control Method

作　　者：魏惠珍[1] 谢菲[2] 饶毅[1] 杜艳龙[1] 金浩鑫[1] 李新南[2] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]江西中医学院,南昌330004

出　　处：《中国实验方剂学杂志》2010年第14期74-77,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家十一五科技支撑计划项目(2006BAI08B03-05)

摘　　要：目的:建立内标多控法测定芸香科植物枳实、枳壳、青皮以及陈皮中黄酮类成分的含量。方法:以柚皮苷为内标,建立柚皮苷与橙皮苷、新橙皮苷的相对校正因子,对其进行耐用性考察;采用内标多控法计算芸香科植物中黄酮类成分的含量,并用夹角余弦法对计算值与外标法的实测值进行比较。结果:流速、柱温、色谱柱以及仪器对相对校正因子无显著影响;采用内标多控法计算芸香科植物黄酮类成分的含量与外标法的实测值没有显著差异。结论:所建立的内标多控法可用于枳实、枳壳、青皮以及陈皮中黄酮类成分的定量分析和质量控制。Objective：To establish the internal standard multi-control method and applied to the determina-tion of flavonoid compounds in rutaceae plant：citrus aurantium,bitter orange Immature,walnut peel and tangerine peel.Method：Naringin as index,establishing the relative correction factor of naringin,hesperidin and neohesperi-din and investigating the durability.Internal standard multi-control method was used to determine the content of fla-vonoid compounds in rutaceae plant,using included angle cosine method to compare the calculated values with measured values by external standard method.Result：The flow rate,column temperature,columns and instruments did not have significant effect on the relative correction factor and there was no significant difference between the calculated values of determination of flavonoid compounds in rutaceae plant by ＂internal standard multi-control method＂and measured values by external standard method.Conclusion：The established internal standard multi-control method can be used to quantitative analysis and quality control of flavonoid compounds in citrus aurantium,bitter orange immature,walnut peel and tangerine peel.

关 键 词：内标多控法 柚皮苷 橙皮苷 新橙皮苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]