高效液相色谱法测定盆炎净片中咖啡酸的含量  

Determination of Caffeic Acid in PYJP by HPLC

作　　者：宁康健[1] 冯惠平[2] 何胜利[2] 

机构地区：[1]安徽科技学院,安徽凤阳233100 [2]天津药业集团新郑股份有限公司,河南新郑451150

出　　处：《中国实验方剂学杂志》2010年第14期80-81,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市卫生局中西医结合资助课题(07018)

摘　　要：目的:应用高效液相色谱法测定盆炎净片(PYJP)中咖啡酸的含量。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),甲醇-磷酸盐缓冲液(23∶77)为流动相,流速1.0 mL.min-1,检测波长323 nm。结果:咖啡酸在0.072 96～0.437 76μg进样量与峰面积呈良好的线性关系(r=0.999 9),平均加样回收率为99.10%,RSD 1.22%(n=5)。结论:该方法测定结果准确、灵敏、重复性好。Objective：To develop an HPLC method for determination of the content of caffeic acid in PYJP.Method：An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-phosphate buffer（23∶ 77）,the flow rate was 1.0 mL.min-1.The UV detection wavelength was at 323 nm.Result：There was a good linear relationship between the concentration of caffeic acid and peak area value when the concentrations of caffeic acid was within the range of 0.072 96-0.437 76 μg（r = 0.999 9）.The average recovery was 99.10%,RSD 1.22%（n = 5）.Conclusion：The method was accurate,sensitive,reproducible and may be used in the determina-tion of caffeic acid in PYJP

关 键 词：盆炎净片 咖啡酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]