实验性大鼠肾草酸钙结石造模方法筛选研究  

Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

作　　者：赖海标[1] 刘朝晖[1] 吴松[1] 欧秀华[2] 顾向明[1] 王书芹[1] 高玉桥[1] 林慧[1] 汪芸[1] 戴卫波[1] 范文昌[1] 丘兰英[1] 林家乐[1] 梅全喜[1] 

机构地区：[1]中山市中医院,广东中山528400 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2010年第14期135-138,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中山市科技计划项目(20071A016);广东省中医药局科技基金项目(2007424)

摘　　要：目的:筛选一种方法简便、稳定、成石效果好的实验性大鼠肾草酸钙结石造模方法。方法:采用几种目前常用的实验性大鼠肾草酸钙结石造模方法(造模Ⅰ组:1%乙二醇饮水+2%氯化铵每天ig 2 mL/只;造模Ⅱ组:1.25%乙二醇+1%氯化铵,ig 20 mL.kg-1.d-1;造模Ⅲ组:每只每天饮用1.25%乙二醇+1%氯化铵10 mL,水30 mL;造模Ⅳ组:1%乙二醇+1%氯化铵自由饮用)进行造模,并进行各组尿液、血液和肾组织中的多项生化指标【尿素氮(BUN)、肌酐(Cr)、尿酸(UA)、无机磷(IP)、钙(Ca2+)、草酸(OX)】检测以及肾脏病理标本观察,综合评价造模效果。结果:造模Ⅰ组的24 h尿液中的BUN,Cr,UA,IP,Ca2+,OX均高于正常对照组(Ⅴ组),且统计学上有极显著性差异(P<0.01);血液中BUN,Cr,UA,IP,Ca2+均高于正常对照组,且统计学上有极显著性差异(P<0.01);肾组织中Ca2+和OX均高于正常对照组,且统计学上有极显著性差异(P<0.01);肾组织可见大量草酸钙结晶沉积。造模Ⅱ组和造模Ⅲ组的多项生化指标结果显示大鼠的肾功能下降,但肾组织中未见结晶体,仅发现肾间质血管充血。造模Ⅳ组大鼠全部死亡。结论:造模Ⅰ组为最佳造模方法。Objective：To select a simple,stable and satisfied experimental model of renal calcium oxalate calculus in rats.Method：After several renal calcium oxalate calculus models currently used（Model group I：administered intragastrically with 1% ethylene glycol free drink ＋ 2% ammonium chloride 2 mL/rat per day;model Group Ⅱ：administered intragastrically with 1.25% ethylene glycol ＋ 1% ammonium chloride 20 mL.kg-1 per day;model group Ⅲ：drank 10 mL 1.25% ethylene glycol ＋ 1% ammonium chloride and 30 mL water per day;model group Ⅳ：drank 1% ethylene glycol ＋ 1% ammonium chloride freely.） experimental rat models were developed.Several biochemical indicators[urea nitrogen（BUN）,creatinine（Cr）,uric acid（UA）,inorganic phosphorus（IP）,calcium（Ca2 ＋）,oxalate（OX）] of urine,blood and nephridial tissue were measured and renal pathological specimens were observed.Then the modeling results were comprehensive evaluated.Result：The 24 h urinary BUN,Cr,UA,IP,Ca2 ＋,OX of model groupⅠwere singnificantly higher than the normal control group（groupⅤ）（P 0.01）;blood BUN,Cr,UA,IP,Ca2 ＋ were significantly higher than the normal control group（P 0.01）;nephridial tissue Ca2 ＋ and OX were significantly higher than the normal control group（P 0.01）;and calcium oxalate crystals were distributed in nephridial tissue.The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats＇ renal function breakdown,and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadn＇t been seen in nephridial tissue.All rats of model group Ⅳ were dead.Conclusion：Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.

关 键 词：草酸钙 肾结石 动物模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]