三黄益肾胶囊对糖尿病大鼠肾脏转化生长因子β1及血管内皮生长因子表达的影响  

Effects of Sanhuang Yishen Capsule on Expression of TGF-β1 and VEGF in Kidney of Diabetic Rats

作　　者：迟秀娥[1] 王元松[1] 田风胜[1] 苏秀海[1] 李文东[1] 王晓蕴[1] 屈会云[1] 

机构地区：[1]河北医科大学附属沧州中西医结合医院内分泌科,河北沧州061001

出　　处：《中国实验方剂学杂志》2010年第14期143-146,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察经验方三黄益肾胶囊对糖尿病早期肾病大鼠的干预作用,探讨其可能的作用机制,为临床防治糖尿病肾病提供可靠的实验依据。方法:糖尿病模型大鼠随机分为4组:模型对照组、三黄益肾低剂量治疗组(0.4 g.kg-1.d-1)、中剂量治疗组(0.8 g.kg-1.d-1)、高剂量治疗组(1.6 g.kg-1.d-1)。另设一组正常对照组。三黄益肾胶囊治疗8周末,观察各组血糖、糖化血红蛋白、肌酐、尿素氮、胆固醇、甘油三酯水平及肾重/体重指数(肾重/体重比值×1000)。采用免疫组化法检测大鼠肾组织中转化生长因子β1(TGF-β1),血管内皮生长因子(VEGF)的表达。结果:经治疗8周后,三黄益肾中、高剂量治疗组与糖尿病模型对照组比较,肾重/体重比值明显减少(P<0.01);血清肌酐、尿素氮水平明显降低(P<0.01)。肾组织中TGF-β1及VEGF表达低于糖尿病模型对照组(P<0.05)。结论:三黄益肾胶囊能够降低TGF-β1,VEGF在肾脏的表达水平,延缓糖尿病大鼠的肾损伤。Objective：To observe and verify the intervention action of Sanhuang Yishen Capsule in rats with early diabetic nephropathy,and to study its possible mechanism,so as to provide experimental evidence for the clinic prevention of diabetic nephropathy.Method：Diabetic rats（n = 13/group） were randomly divided into diabetes model group,low-dose Sanhuang Yishen Capsule treated group（0.4 g.kg-1.d-1）,middle-dose Sanhuang Yishen Capsule treated group（0.8 g.kg-1.d-1）,high-dose Sanhuang Yishen Capsule treated group（1.6 g.kg-1.d-1）.Ten SD rats were selected as control group.At the end of the 8th week,we detected blood glucose,glycosylated hemoglobin,creatinine,urea nitrogen,triglyceride,cholesterol and kidney weight/body weight index.The expression of renal TGF-β1 and VEGF were assessed by immune-histochemistry.Result：After treatment of 8 weeks,compared with the diabetes model group the middle-dose and the high-dose Sanhuang Yishen Capsule groups showed obviously decreases in kidney weight/body weight index and the level of serum creatinine,urea nitrogen（P 0.01）.The expression of TGF-β1 and VEGF in kidney of middle-,high-dose Sanhuang Yishen Capsule treated group rats was lower than that of the diabetes model group（P 0.05）.Conclusion：Sanhuang Yishen Capsule can reduce the expression of TGF-β1 and VEGF in the kidney of diabetic rats and delay of renal injury in diabetic rats.

关 键 词：糖尿病肾病 三黄益肾胶囊 转化生长因子Β1 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]