3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-кB信号转导通路的影响  

Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats

作　　者：唐丹丽[1] 刘寨华[2] 隋宇[1] 佟林[3] 张华敏[3] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院中医基础理论研究所,北京100700 [3]中国中医科学院中医药信息研究所,北京100700

出　　处：《中国实验方剂学杂志》2010年第14期161-164,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30701066)

摘　　要：目的:观察中医不同治法方药对大鼠心肌缺血再灌注损伤(I/R)核因子-кB(NF-кB)信号转导通路的影响。方法:将60只SD大鼠随机分为5组:假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制I/R模型,应用Western blotting及RT-PCR法检测各组大鼠心肌核因子IкB诱导激酶(NIK)、IкB激酶β(IKKβ)、核转录因子抑制蛋白(IкBα)及NF-кBp65 mRNA的表达。结果:与模型组比较,各治疗组均能降低心肌NIK,IKKβ,NF-кBp65 mRNA水平并上调IкBα蛋白表达(P<0.01)。结论:祛痰宽胸法、活血化瘀法及痰瘀同治法均对I/R大鼠有保护作用,其主要作用机制可能是通过抑制NIK,IKKβ蛋白表达,减少IкBα蛋白的磷酸化降解而增加其蛋白水平,从而抑制NF-кB的过度活化,发挥其对缺血再灌注损伤心肌的保护作用。Objective：To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion（I /R） injury in rats.Method：Sixty SD rats were divided into 5 groups randomly,sham-operated group：model group,removing phlegm and enlarging chest group,activating blood and dissolving stasis group,treating both phlegm and blood stasis group.The model of I /R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.NIK,IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method.And the levels of NF-кBp65 mRNA were determined with RT-PCR.Result：Compared with model group,the expression of NIK,IKKβ and NF-кBp65 mRNA were lower,and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments（P 0.01）.Conclusion：The removing phlegm andenlarging chest method,activating blood and dissolving stasis method,treating both phlegm and blood stasis method can protect myocardium from I /R.The mechanism of action was related to inhibiting the protein expression of NIK,IKKβ and enhancing the expression of IкBα,restraining the activation of NF-кB.

关 键 词：不同中医治法 心肌缺血再灌注损伤 信号转导通路 

分 类 号：R285.5[医药卫生—中药学]