采血后不同时间内检测血小板聚集功能分析  

The Detemination of Blood plateler Aggregation Function in Different times after Blood collection

作　　者：凌利芬[1] 朱新建[1] 王海[2] 罗敏[1] 黄晓丽[1] 韦宁[1] 陆学东[1] 

机构地区：[1]广东医学院附属深圳第四人民医院检验科,518033 [2]中国人民解放军总医院临床检验科,北京100853

出　　处：《国际检验医学杂志》2010年第10期1099-1100,共2页International Journal of Laboratory Medicine

摘　　要：目的通过检测血小板聚集功能,检测血小板功能缺陷,以及监测抗血小板药物使用。方法采用比浊法,将富血小板血浆(PRP)置于比色管中,加入诱聚剂后,用涂硅的小磁粒进行搅拌,血小板逐渐聚集,血浆浊度降低,透光度增加,记录此变化,形成血小板聚集的动态曲线。结果通过血浆浊度的变化,记录血小板最大聚集率,采血后不同时间内检测血小板聚集率结果有差异。结论为了准确地检测人体血小板的缺陷或监测抗血小板药物疗效,需要在采血后3h内进行血小板聚集试验。Objective By measurement platelet aggregation,to test platelet dysfunction,and monitor anti-platelet drug use. Methods Using turbidimetric method,platelet rich plasma（PRP） was placed in colorimetric tube. Then add poly inducing agent and stir small magnetic particles coated with silicon,platelet gradually aggregated and plasma turbidity reduced and light transmission increased. This change was recorded and forming the dynamic curve of platelet aggregation. Results Recorded the maximum platelet aggregation rate by the changes of plasma turbidity, It showed the piatelet aggregation rates were different with the different time of blood collectionConclusion For more accurate result,blood platelet aggregation need to be tested within 3h after blood collection.

关 键 词：血小板聚集 散射测浊法和比浊法 血样采集 

分 类 号：R446.1[医药卫生—诊断学]