ELAC2对乳腺癌细胞MCF-7浸润转移和放射敏感性的影响  

Effects of ELAC2 on the invasion,migration and radio-sensitivity of human breast cancer cell MCF-7

作　　者：宁萍[1] 焦旸 朱巍[1] 徐加英[1] 胡旭东[1] 樊赛军[1] 

机构地区：[1]苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室,苏州215123

出　　处：《辐射研究与辐射工艺学报》2010年第5期271-276,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30701001);苏州大学医学发展基金(EE126708)资助

摘　　要：为了探讨ELAC2对乳腺癌细胞MCF-7浸润转移和放射敏感性的影响,利用脂质体转染法将ELAC2基因转入MCF-7细胞,Western blot法检测ELAC2、cyclinB、CDC2和P53蛋白表达水平,细胞划痕法和Boyden小室法检测细胞浸润转移能力,集落形成法检测细胞存活分数,流式细胞仪检测细胞周期。结果表明,成功构建了ELAC2高表达的MCF-7/ELAC2细胞株;ELAC2高表达明显抑制细胞的浸润和转移;细胞受X线照射后,ELAC2高表达使细胞放射敏感性增强,G2期细胞比例增多,同时cyclinB表达降低,P53和Tyr-15磷酸化CDC2表达升高。提示ELAC2高表达可明显抑制细胞浸润和转移;ELAC2高表达使细胞辐射敏感性增加很可能与下调cyclinB、上调P53和Tyr-15磷酸化CDC2蛋白水平有关。To study the effects of ELAC2 on the invasion, migration and radio-sensitivity of human breast can-cer cell MCF-7, ELAC2 genes were transfected into MCF-7 using Lipofectamine transfection assay.Western blot assay was used to detect the protein expression of ELAC2, cyclinB, CDC2 and P53 while wound healing assay and Boyden chamber assay were performed to determine cell invasion and migration, and colony formation assay and flow cytometry assay were used to detect cell surviving fraction and cell cycle.The results show that MCF-7/ELAC2 cell lines with overpression of ELAC2 were successfully established.The overexpression of ELAC2 could obviously inhibit invasion and migration of MCF-7 cells.The overexpression of ELAC2 enhanced the radiosensitivity of MCF-7 cells after x-ray exposure and caused increased ratio of G2 phase.At the same time cyclinB protein expression decreased and the expressions of P53 and Tyr-15 phosphorylated CDC2 up-regulated.The results suggest that the overexpression of ELAC2 may obviously inhibit invasion and migration of MCF-7 cells, and may result in radio-sensitivity enhancement of MCF-7 cells to X ray, which could be associated with either down-regulation of protein expression of cyclinB or up-regulations of protein P53 and Tyr-15 phosphorylated CDC2.

关 键 词：ELAC2 乳腺癌细胞MCF-7 浸润转移 放射敏感性 

分 类 号：R811.5[医药卫生—放射医学] R737.9[医药卫生—临床医学]