γ射线辐照降解乳酸左氧氟沙星及产物抑菌活性分析  

γ-radiolytical degradation of levofloxacin lactate and the activity of the byproducts

作　　者：曹冬梅[1] 张晓红[1] 关钰[1] 朱伟[1] 张海黔[1] 

机构地区：[1]南京航空航天大学,核技术及应用专业南京211100

出　　处：《辐射研究与辐射工艺学报》2010年第5期277-281,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(GrantNo20975052);江苏省自然基金(SBK200922574);南京航空航天大学研究生创新基地(实验室)开放基金2010资助

摘　　要：研究了γ射线辐照下10μg/mL乳酸左氧氟沙星的降解,空气条件下当剂量达到1kGy时,降解率达到99%,而G值则随着剂量增加而降低;通过高效液相色谱-质谱联用仪(LC-MS)分析,推测出5种主要的降解产物(m/z346、330、318、302、274)和最可能的降解途径,并与氮气条件下的降解进行比较,探讨初步降解机理;对大肠杆菌在含有乳酸左氧氟沙星溶液的营养肉汤中生长状况分析可知,产物为m/z346的分子仍然具有活性成分,可以抑制大肠杆菌的生长,仅当剂量大于3kGy活性才完全消失。因此3kGy应为乳酸左氧氟沙星的最佳辐照剂量。辐照技术可以很有效地降解抗生素,确定辐照剂量时需要把降解产物活性考虑在内。Recently antibiotics wastewater in environment has induced the increment of bacterial resistance.This paper is to investigate the gamma radiolysis of wastewater containing 10 μg/mL levofloxacin lactate （LVF）.It has been found that the antibiotic was removed more than 99% with 1 kGy under air while the G-value decreased with the dose increment.Five main degraded products （m/z 346, 330, 318, 302, 274） and the most probable radiolysis pathway were identified by liquid chromatography-mass spectrometry （LC-MS）.Compared with the degradation under N2, the radiolytical mechanism was suggested.In the active assay, 2 μg/mL was the inhibitory concentration.Compared with the concentration of 4 μg/mL, the minimal inhibitory concentration （MIC）, degraded products analysis can be suggested that the byproduct m/z 346 might have the anti-E.coli activity.It has been shown that 3 kGy is the appropriate dose for the radiolytical treatment of LVF.To sum up, the gamma radiation technique is an effective method for decomposing antibiotics, and it is necessary to take the activity of degraded products into consideration.

关 键 词：Γ射线 乳酸左氧氟沙星 辐照降解 LC-MS 活性 

分 类 号：O644[理学—物理化学] O657[理学—化学]