盐生植物小花碱茅K^+通道PtAKT1基因片段的克隆及序列分析  

Cloning and sequence analysis of K^+ channel PtAKT1 gene fragment from halophyte (Puccinellia tenuiflora)

作　　者：郭强[1] 周向睿[1] 王沛[1] 张金林[1] 包爱科[1] 伍国强[1] 王锁民[1] 

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草地学报》2010年第5期683-688,共6页Acta Agrestia Sinica

基　　金：国家973项目(2007CB108901);863计划项目(2006AA10Z126);国家自然科学基金(30770347)(30700562)资助

摘　　要：为研究小花碱茅(Puainellia tenuiflora)K+/Na+选择吸收分子机制,根据已报道的其他植物AKT1基因的保守序列设计一对简并性引物,以小花碱茅根部总RNA为模板,采用RT-PCR方法克隆出AKT1基因,以期为小花碱茅AKT1全长基因的克隆、表达调控及其作物改良的研究奠定基础。序列分析结果表明:该基因长度大约为1019 bp,编码339个氨基酸;所得序列与GenBank中注册的高等植物AKT1基因序列的同源性均在85%以上,与其他AKT1的氨基酸序列同源性达73%以上。Degenerate primers were designed based on the conserved sequences of the AKT1 genes from other plants for investigating molecular mechanisms of K^＋/Na^＋ selective absorption in P.tenuiflora.Total RNA was extracted from the roots of Puccinellia tenuiflora to obtain an AKT1 gene fragment by reverse transcription polymerase chain reaction（RT-PCR）.The sequencing result shows that the AKT1 gene fragment（about 1019 bp） from Puccinellia tenuiflora encodes 339 amino acids.Homology comparison show that the AKT1 gene fragment contains over 85% nucleotide and 73% amino acid homology when compared with other plants in the GenBank having an AKT1 gene.These results offer further understanding for full-length AKT1 gene cloning,gene expression regulation and crop improvement.

关 键 词：小花碱茅 AKT1基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学] Q945.12