大花金挖耳细胞悬浮培养及其胞内代谢产物化感潜力的研究  

Allelopathy Potential of Intracellular Secondary Metabolic Products and Cell Suspension Culture of Carpesium macrocephalum Franch.et Sav.

作　　者：李玉平[1,2] 张妮[2] 陈晓旭[2] 冯俊涛[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心/陕西省生物农药工程技术研究中心,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《草地学报》2010年第5期708-713,共6页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(30971934);生物源农药创制与技术集成及产业化开发项目[公益性行业(农业)科研专项(200903052)];西北农林科技大学博士科研启动费(01140504)资助

摘　　要：为了优化大花金挖耳（Carpesium macrocephalum Franch.et Sav.）细胞悬浮培养的条件,评价其胞内代谢产物的化感潜力。本文研究了无机盐浓度不同的MS,1/2MS,B5,1/2B5,NT和1/2NT等6种培养基及NT中N,P,Ca,Mg,K等5种大量元素含量对大花金挖耳悬浮培养细胞生物合成黄酮的影响,并采用微量活性测定法测定了其细胞培养物的化感潜力。结果表明：大花金挖耳细胞悬浮培养的最优基本培养基是NT,NT中总氮浓度为50.62 mmol·L^-1,NH4^＋∶NO3^-为2∶3时,黄酮含量最高为1.38%,H2PO4^-,Ca^2＋,Mg^2＋,K^＋等离子依次在5.00,1.50,0.50-1.00,14.39-27.81 mmol·L^-1范围内有利于大花金挖耳悬浮培养细胞的生长和黄酮合成;大花金挖耳悬浮培养细胞的粗提物对供试的紫花苜蓿（Medicago sativaL.）、刺儿菜（Cephalanoplos segetum（Bge.）Kitam.）、反枝苋（Amaranthus retroflexus L.）、小白酒草（Conyza canadensis（L.）Cronq.）和小麦（Triticum aestivumL.）等5种植物幼苗根生长均具有抑制作用,毒力依次为10.66,12.32,15.85,102.16,210.96 mg·mL^-1。Allelopathic effects of intracellular secondary Carpesium macrocephalum Franch. et Sav. are not well metabolic products and cell suspension culture of reported. In order to optimize cell suspensing culture conditions of C. macrocephalum and to investigate the allelopathy of extracts from C. macrocephalum suspension cells, the effects of different factors on the cell growth and the accumulation of total flavonoids were studied during C. macrocephalum suspension culture under the MS, 1/2MS, BS, 1/2B5,NT and 1/ 2NT media, respectively. Results showed that NT medium was suitable for cell cultures according to cell growth and the high content of flavonoid. Effects of five macronutrients on cell growth and flavonoid synthesis were significantly different between treatments （P〈0.05）. The highest flavonoid content （1.38 %） was obtained when the total nitrogen concentration was 50.62 mmol · L^- 1 and the ratio of NH4^＋ / NO; in the medium was 2/3. The best concentrations for H2PO4^- ,Ca^2＋ ,Mg^2＋ and K^＋ were 5.00 mmol · L ^-1,1.50 mmol · L^-1 ,0.50-1.00 mmol· L^-1 ,14.39-27.81 mmol · L^-1 , respectively. Also, ethanol exiracts of eell cultivation products from C. macrocephalum had herbicidal activity against 5 tested plant seeds. EC50 values of ethanol extracts to Medicago sativa L. ,Cephalanoplos segetum （Bge.） Kitam. ,Amaranthus retroflexus L. ,Conyza canadensis （L.）Cronq. and Triticum aestivum L. were 10.66,12.32,15.85,102.16, 210.96 mg·mL^-1 , respectively.

关 键 词：大花金挖耳 细胞培养 生物合成 黄酮 化感 

分 类 号：Q813.11[生物学—生物工程] Q251