玉米S-腺苷甲硫氨酸合成酶基因(SAMS)逆境胁迫下的表达分析  

Expressional analysis of SAMS gene from Zea mays under abiotic stresses

作　　者：赵晋锋 余爱丽[2] 朱晶莹[2] 王高鸿 赵根友 

机构地区：[1]山西农科院谷子研究所,山西长治046011 [2]河北农业大学生命科学学院,河北保定071000

出　　处：《河北农业大学学报》2010年第5期13-17,共5页Journal of Hebei Agricultural University

基　　金：山西农科院博士科研启动基金(YBSJJ1002)

摘　　要：玉米是对干旱、低温、高温等逆境较为敏感的重要禾谷类作物,发掘玉米逆境相关基因及对逆境信号途径研究具有重要学术意义与应用价值。S-腺苷甲硫氨酸合成酶(S-adenosyl-L-metnionine synthetase,SAMS)是植物代谢中一个关键酶,催化合成腺苷甲硫氨酸(SAM)。试验在前期克隆玉米SAMS基因基础上,顺式元件分析发现SAMS启动子区域存在ABA(ABRE)、干旱(MBS)、逆境应答(TC-rich repeats)等顺式作用元件;荧光实时定量PCR表达分析发现干旱、高盐、ABA处理下基因表达量上调,在低温、高温处理下基因表达量变化不大。结果表明:玉米S-腺苷甲硫氨酸合成酶基因响应植物逆境应答,为SAMS基因参与植物逆境信号通路及其功能研究提供理论依据。As an important cereal crop,maize is sensitive to drought,cold,heat and other stresses.Therefore,development of new maize stress related genes and study of stress signaling pathway are important in terms of academic value and application.S-adenosyl-L-metnionine synthetase（SAMS） is a key enzyme in plant metabolize.The syntheses of S-adenosyl-L-methionine（SAM） were catalyzed by SAMS.Under the basic research of prophase,cis-element analysis identified ABA,drought and other stresses core sequence within the promoter region of SAMS gene.Real-time PCR results indicated that the expression of SAMS gene was up-regulated under the drought,salt and ABA treatment,but no obvious change was detected under the cold and heat treatment.This study demonstrated maize SAMS gene is responsive to abiotic stresses,which provides theoretical groundwork for further exploration into the function of SAMS in maize.

关 键 词：玉米 SAMS 表达分析 逆境胁迫 

分 类 号：S183[农业科学—农业基础科学]