17个粗山羊草品种(系)抗叶锈基因的鉴定  

Identification of wheat leaf rust resistance gene in 17 Aegilops tauschii lines

作　　者：陈玉婷[1] 魏学军[1] 高峰[2] 胡亚亚[1] 张娜[1] 王晓辉[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]河北农业大学党委办公室,河北保定071001

出　　处：《河北农业大学学报》2010年第5期34-39,共6页Journal of Hebei Agricultural University

基　　金：国家"十一五"支撑计划(2006BAD08A05)

摘　　要：为鉴定17个粗山羊草品种(系)中可能含有的抗叶锈病基因,用10株具有不同毒力的小麦叶锈菌混合菌对17个粗山羊草品种进行成株期抗叶锈性鉴定,筛选出7个在田间对叶锈菌有抗性的材料。用抗叶锈基因Lr1、Lr9、Lr19、Lr21、Lr24、Lr28、Lr29和Lr34的STS、SCAR或CAPS标记对这些品种进行分子辅助鉴定。初步明确粗山羊草CN40033和CN30823中可能含有Lr1基因;CN42471中可能含有Lr9基因;CN30942中可能含有Lr21;供试的17个粗山羊草品种中都不含有Lr19、Lr24、Lr28、Lr29和Lr34。The objective of this research is to identify the wheat leaf rust resistance genes in the Aegilops tauschii.Mixture of ten wheat leaf rust pathotypes conferring different virulence to wheat was used to identify the wheat leaf resistance genes of 17 lines of A.tauschii.Seven of the eleven accessions were resistant to the test pathotypes of Puccinia triticina at adult stage.A series of STS,SCAR and CAPS markers linked to resistance genes of Lr1,Lr9,Lr19,Lr21,Lr24,Lr28,Lr29 and Lr34 were used for marker assisted identification.The results showed that Lr1 might be present in CN40033 and CN30823,and Lr9 identified in CN42471and Lr21 was found in CN30942.Five genes Lr19,Lr24,Lr28,Lr29 and Lr34 were not identified in the 17 lines.

关 键 词：小麦叶锈病 粗山羊草 抗叶锈病基因 分子标记辅助鉴定 

分 类 号：S435.121.43[农业科学—农业昆虫与害虫防治]