Elementary study of differential expression of total soluble proteins in different life phases of Porphyra yezoensis  

不同时期和生长状态条斑紫菜总可溶性蛋白差异表达初探(英文)

作　　者：孟春晓[1] 高政权[1] 王依涛[1] 罗韬[1] 叶乃好[2] 

机构地区：[1]山东理工大学生命科学学院,山东淄博255049 [2]中国水产科学院黄海水产研究所,山东青岛266071

出　　处：《Marine Science Bulletin》2010年第2期32-37,共6页海洋通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 40706050, 40706048 and 30700619);the National Science & Technology Pillar Program (No. 2006BAD01A13 and 2008BAC49B04);National special fund for transgenic project (No. 2009ZX08009-019B);Natural Science Foundation of Shandong Province (No. 2009ZRA02075);and Qingdao Municipal Science and Technology plan project (No. 09-2-5-8-hy)

摘　　要：Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.用SDS-PAGE技术研究了不同时期条斑紫菜样品、高温下培养的丝状体以及不同季节收获的叶状体的总可溶性蛋白。结果表明,这些样品总可溶性蛋白的表达数量和种类相差很大。壳孢子中总可溶性蛋白的表达数量和种类明显比丝状体中多,但比膨大丝状体中少;高温条件下培养的丝状体比正常条件下培养的丝状体表达更多数量和种类的可溶性蛋白;所有样品中,以叶状体表达的溶性蛋白的表达数量和种类最多;上年收获的与本年度不同季节收获的叶状体在蛋白质表达图谱上差别很小。

关 键 词：Porphyra yezoensis total soluble proteins differential expression 

分 类 号：S968.431[农业科学—水产养殖]