旋毛虫肌幼虫在不同培养基中发育情况的观察  

Observation of culture conditions for Trichinella spiralis muscle larvae in vitro

作　　者：王莉 崔晶[1] 王书伟[1] 王中全[1] 

机构地区：[1]郑州大学医学院寄生虫学教研室,河南郑州450052

出　　处：《中国病原生物学杂志》2010年第10期746-748,728,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.30972579);高校博士点专项科研基金项目(No.20094101110004);郑州大学研究生科学研究基金资助项目(No.A119)

摘　　要：目的观察旋毛虫肌幼虫在不同培养基中的发育情况。方法将旋毛虫肌幼虫接种在不同培养基中于37℃5%CO2条件下培养18 d,观察幼虫开始蜕皮的时间和蜕皮率,并测量幼虫长度。结果肌幼虫在RPMI-1640、含5%胎牛血清（FBS）的RPMI-1640培养液、PBS和生理盐水中开始蜕皮的时间分别为15、23、19和37 h;肌幼虫在RP-MI-1640与5%FBS＋RPMI-1640中分别蜕皮1~4层和1~2层,在PBS与生理盐水中仅蜕皮1层。培养5 d后,RP-MI-1640中培养幼虫的蜕皮率（77.94%）显著高于在其他3种培养液培养的幼虫蜕皮率（39.83%、44.64%和8.84%）（Z1640＋5%FBS=-2.268,ZPBS=-2.641,Zsaline=-6.826,P〈0.05）;培养18 d后,在RPMI-1640中蜕2~4层皮的幼虫蜕皮率（25.6%、1.32%及0.26%）显著高于在5%FBS＋RPMI-1640中培养的幼虫蜕皮率（0.45%、0和0）（Z2=-16.111,Z3=-7.991,Z4=-3.885,P〈0.05）;在2种培养液中培养1~18 d的幼虫长度差异无统计学意义（t=0.138,P〉0.05）,幼虫长度均随培养时间的延长而减小（F1640=54.639,P〈0.05;F1640＋5%FBS=26.928,P〈0.05）。结论绝大多数旋毛虫肌幼虫在RPMI-1640与5%FBS＋RPMI-1640培养液中不能蜕下完整表皮,不能发育到成虫阶段。Objective To observe the development of Trichinella spiralis muscle larvae in vitro in different media.Methods The larvae were inoculated in different media and cultured at 37 ℃ in 5%CO2 for 18 days.The molting time and rate of the cultured larvae were observed,and larval length was measured.Results The time when the larvae cultured in RPMI-1640,RPMI-1640＋5% fetal bovine serum（FBS）,PBS and saline began to molt was 15,19,23 and 37 h,respectively.Larvae with 1-4 and 1-2 sheath（s） were formed in RPMI-1640 and RPMI-1640 ＋5% FBS medium.Only a single sheath was observed when larvae were cultured in PBS and saline.The molting rate of larvae cultured in RPMI-1640 medium（77.94%） was significantly higher than that in 3 other kinds of media（39.83%,44.64%,and 8.84%） after culturing for 5 days（Z1640＋5%FBS=-2.268,ZPBS=-2.641,Zsaline=-6.826,P0.05）.After culturing for 18 days,the molting rates of larvae with 2-4 sheaths cultured in RPMI-1640 medium（25.6%,1.32% and 0.26%） were significantly higher than that in RPMI-1640＋5% FBS medium（0.45%,0 and 0）（Z2=-16.111,Z3=-7.991,Z4=-3.885,P0.05）.There was no statistically significant difference in the length of larvae cultured in RPMI-1640 and RPMI-1640＋5% FBS medium for 1-18 days（t=0.138,P0.05）,and the length decreased as the culturing time increased（F1640=54.639,F1640＋5%FBS=26.928,P0.05）.Conclusion The majority of T.spiralis muscle larvae cultured in RPMI-1640 and RPMI-1640＋5% FBS medium were unable to completely shed the old cuticle and develop into adults.

关 键 词：旋毛虫 肌幼虫 发育 体外培养 蜕皮 

分 类 号：R383.15[医药卫生—医学寄生虫学]