rSj26-Sj32-Immunogold-IgG-Dot-ELISA用于慢性日本血吸虫病的诊断研究  

Study on the diagnosis of chronic schistosomiasis japonicum using rSj26-Sj32-immunogold-IgG-dot-ELISA

作　　者：蔡世飞[1] 李文桂[1] 王敏[1] 周必英[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国病原生物学杂志》2010年第10期754-757,761,共5页Journal of Pathogen Biology

基　　金：重庆市科委地方病重大专项基金(No.2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的探讨rSj26-Sj32-Immunogold-IgG-Dot-ELISA用于慢性日本血吸虫病的诊断价值。方法分别以rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)作为包被抗原,通过Immunogold-IgG-Dot-ELISA检测慢性日本血吸虫病患者血清抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎、肺结核病人血清及健康人血清作为对照。结果 rSj26-Sj32-Immunogold-IgG-Dot-ELISA检测日本血吸虫抗体的敏感性和特异性分别为95.00%和97.67%,且与华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎和肺结核患者血清均无交叉反应;诊断慢性日本血吸虫病的阳性预告值、阴性预告值分别为97.44%和95.45%,诊断效率为96.39%,与SjAWA-Immunogold-IgG诊断效率(96.39%)相同。结论 rSj26-Sj32-Immunogold-IgG-Dot-ELISA法可用于慢性日本血吸虫病的诊断。Objective To study the value of rSj26-Sj32-Immunogold-IgG-Dot-ELISA in the diagnosis of chronic schistosomiasis japonicum.Methods IgG antibodies were detected in sera from patients with chronic schistosomiasis japonicum using rSj26-Sj32 fusion protein and SjAWA in immunogold-IgG-Dot-ELISA.The sera of patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or pulmonary tuberculosis and the sera of healthy people served as a control.Results rSj26-Sj32-immunogold-IgG-dot-ELISA had a sensitivity and specificity of 95.00% and 97.67%,respectively,and there was no cross-reaction with the sera from patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or pulmonary tuberculosis.The technique had a positive predictive value and negative predictive value of 97.44% and 95.45%,respectively,and its diagnostic efficiency was 96.39%,the same as that of SjAWA-Immunogold-IgG.Conclusion rSj26-Sj32-immunogold-IgG-dot-ELISA may be used in the immunodiagnosis of chronic schistosomiasis japonicum.

关 键 词：rSj26-Sj32融合蛋白 SjAWA 免疫诊断 DOT-ELISA 

分 类 号：R532.21[医药卫生—内科学]