两种试剂盒快速检测斯氏狸殖吸虫抗体的研究  

Comparison of two reagent kits for the rapid detection of Pagumogonimus skrjabini antibody

作　　者：朱名胜[1] 朱敬[2] 王绍基[1] 宋明华[1] 

机构地区：[1]湖北医药学院寄生虫学教研室,湖北十堰442000 [2]湖北医药学院生物学教研室,湖北十堰442000

出　　处：《中国病原生物学杂志》2010年第10期762-763,共2页Journal of Pathogen Biology

基　　金：日本健康科学基金资助项目(JHSF,HS189)

摘　　要：目的观察肺吸虫病金标渗滤试剂盒(DIGFA kit)和酶联免疫吸附试验(ELISA)试剂盒检测斯氏狸殖吸虫抗体的敏感性和特异性。方法采用DIGFA kit和ELISA检测斯氏狸殖吸虫病流行区人群和病鼠血清抗体。结果 DIGFA和ELISA试剂盒检测斯氏狸殖吸虫病流行区人群和病鼠血清特异性抗体阳性率均为5.08%(54/1062)和100%(40/40);用两种试剂盒检测正常大鼠、旋毛虫病大鼠、血吸虫病兔和蛔虫病病人血清,除1例血吸虫病兔血清DIGFA出现阳性反应外,其他血清两种方法均为阴性。大鼠感染斯氏狸殖吸虫后第2周用两种方法检测抗体,阳性率分别为20.00%(8/40)和27.50%(11/40),第4周阳性率均达100%,并持续至第8周。结论 DIGFA试剂盒具有与ELISA试剂盒相同的敏感性和特异性,且有操作快速、简便、直观等特点,适合在基层和斯氏狸殖吸虫病流行地区推广使用。Objective To explore the sensitivity and specificity of using a dot-immunogold filtration assay（DIGFA） and ELISA to detect the anti-Pagumogonimus skrjabini antibody.Methods DIGFA and ELISA were used to test for anti-P.skrjabini antibody in sera from the population in an area where paragonimiasis was prevalent and infected rats.Results Anti-P.skrjabini antibody was detected in sera from the population in an area where paragonimiasis was prevalent and infected rats at a rate of 5.08% and 100%,respectively.Compared to results from healthy rats,sera from rats with trichinosis,rabbits with schistosomiasis,and patients with ascariasis tested negative according to the two methods except for a positive result in DIGFA in a rabbit with schistosomiasis.The antibody was detected 2 weeks post-infection in lab animals at a rate of 20.00% and 27.50%,respectively,and at 4 weeks that rate reached 100% and remained so for 8 weeks.Conclusion The DIGFA reagent kit has the same sensitivity and specificity as ELISA.Specifically,it is rapid,easy to use,and inexpensive,so further use of this kit is warranted in areas where paragonimiasis is prevalent.

关 键 词：试剂盒 斯氏狸殖吸虫 检测 

分 类 号：R383.23[医药卫生—医学寄生虫学]