乙型肝炎病毒相关性肾炎患者病毒基因型及变异的临床分析  

Clinical analysis of HBV genotypes and mutations in patients with hepatitis B virus-associated glomerulonephritis

作　　者：孙莉静[1] 郭志勇[1] 崔若兰[1] 许静[1] 

机构地区：[1]第二军医大学附属长海医院肾内科,上海200433

出　　处：《中国病原生物学杂志》2010年第10期779-781,共3页Journal of Pathogen Biology

摘　　要：目的检测乙型肝炎病毒相关性肾炎(HBV-GN)患者的HBV基因型,探讨HBV S区、P区变异与HBV-GN病情变化的相关性。方法对10例确诊HBV-GN患者取血,抽提全基因组DNA,DNA测序仪检测HBV P区、S区序列,进行病毒基因分型,并查找突变位点。结果 10例HBV-GN患者检出HBV基因型C型9例(占90%),B型1例(占10%);出现YVDD变异1例,同义突变1例,各占10%。结论本组HBV基因型以C型为主,发生变异少,未发现HBV变异与HBV-GN病情变化的相关性。Objective To determine the HBV genotype in patients with hepatitis B virus-associated glomerulonephritis（HBV-GN） and explore the relationship between clinical outcomes and HBV mutation in the S and P regions.Methods The complete genomic sequences of HBV from 10 HBV-GN patients were determined by direct sequencing of polymerase chain reaction products.Various point mutations in the S and P regions of HBV were detected in all patients.Results The HBV genotype in HBV-GN patients was mainly the C genotype.Only 1 patient was reported to have the B genotype.The incidence of HBV mutations was very low;only one YMDD mutation was detected from serum specimens.One patient had a synonymous mutation,i.e.no changes in the amino acid sequence.Conclusion Results suggested that the HBV genotype was mainly C,and no relationship between HBV gene mutations and the development of HBV-GN was found.

关 键 词：乙型肝炎病毒相关性肾炎 病毒变异 临床研究 

分 类 号：R373.21[医药卫生—病原生物学]