酮胺氧化酶法测定糖化白蛋白的评价  

The evaluation of Ketoamine oxidase method to determinate glycated albumin

作　　者：贾珂珂[1] 李国权[1] 张捷[1] 

机构地区：[1]北京大学第三医院检验科,北京100191

出　　处：《中国实验诊断学》2010年第10期1620-1623,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的对酮胺氧化酶法测定糖化白蛋白(GA)进行方法学评价。方法分析及评价Lucica GA-L试剂盒的精密度、稀释线性、回收率、干扰因素以及试剂稳定性。比较GA与糖化血红蛋白(HbA1c)的相关性,GA与果糖胺(GSP,NBT法)的相关性。比较溴甲酚绿法(BCG)和溴甲酚紫法(BCP)测定白蛋白的相关性。结果该试剂精密度高,批内CV<1.4%,批间CV<2.4%;稀释线性良好;GA的平均回收率为96.74%,ALB的平均回收率为96.73%;葡萄糖、乳糜、胆红素对酶法检测GA无明显干扰,但是血红蛋白浓度在1.82 g/L以上时,对结果的影响超过10%。试剂可稳定4周以上。GA与HbA1c相关性良好(r=0.8940,P<0.001)。BCG法测定白蛋白的值(-x±s,37.1±8.4 g/L)较BCP法(-x±s,33.3±10.0 g/L)高,二者相关性良好(r=0.9908,P<0.001)。当ALB>30 g/L时,GA与GSP相关良好(r=0.8474,P<0.001);当ALB<30 g/L时,GA与GSP相关性差(r=0.5697,P<0.05)。结论该方法分析性能良好,能定量分析糖化白蛋白,并计算其与白蛋白的比值,受到的影响因素较少,能较好的监测短期内平均血糖水平。Objective To evaluate Ketoamine oxidase method of determinating glycated albumin（GA）.Methods To evaluate Lucica GA-L kit by studying the imprecision,dilution linearity,recovery,interference factors,and reagent stability.Analyze the correlation between GA and glycated hemoglobin（HbA1c）;GA and fructosamine（GSP,NBT method）.Compare bromocresol green method（BCG） with bromocresol purple method（BCP） of albumin determination.The results showed good precision,within-run CV 1.4%,between-run CV 2.4%;good linearity;the average recovery rate of 96.74% for GA,and the average recovery rate of 96.73% for ALB.Glucose,chyle and bilirubin had no significant interference on GA,but the hemoglobin affected the results of over 10% when the concentration was over 1.82 g/L.Results was stable for more than 4 weeks.It showed excellent correlation（r=0.894 0,P0.001） between GA and HbA1c.Determination of albumin values with BCG method（±s,37.1 ±8.4 g/L） were higher than that of BCP method（±s,33.3±10.0 g/L）,but with excellent correlation（r=0.990 8,P0.001）.When ALB concentration was over 30 g/L,GA was well correlated to GSP（r=0.847 4,P0.001）;When ALB concentration was less than 30 g/L,they were in poor correlation（r=0.569 7,P0.05）.Conclusion This method of GA determination shows good performance,and it can calculate the ratio of GA and albumin.There are fewer interference factors affecting this method.This method is a good index to monitor short-term average blood glucose levels.

关 键 词：糖化白蛋白 糖尿病 糖化血红蛋白 果糖胺 

分 类 号：R587.1[医药卫生—内分泌]