中国美利奴羊外周免疫器官cDNA文库的构建及鉴定  

Construction and Characterization of cDNA Library from Peripheral Immune Organ of Merino Sheep

作　　者：李鑫[1] 邹毅辉[1] 杨晓亮[1] 李桂芳[1] 李峰[1] 陈芳[1] 石国庆[2] 高剑峰[1] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]新疆农垦科学院畜牧兽医所,石河子832000

出　　处：《石河子大学学报（自然科学版）》2010年第5期529-533,共5页Journal of Shihezi University(Natural Science)

基　　金：科技部重大国际合作项目(2006DFB33750)

摘　　要：为了筛选中国美利奴羊与免疫反应相关的的功能基因并研究这些基因的功能,构建了以美利奴羊外周免疫器官的cDNA文库。通过提取总RNA,分离纯化mRNA并以其为模版参照ZAP Express cDNA Synthesis Kit说明书构建cDNA文库。结果表明:该文库初级库容达到了3.28×105pfu,扩增库滴度为3.8×108pfu/mL,重组率为97.7%。插入片段介于0.5至2.3 kb之间,平均大小为1.5 kb。该文库的成功构建为后续研究奠定了分子基础。A cDNA library from peripheral immune organs of Merino sheep was constructed to screen genes related to Immunal Reaction and explore their functions.Total RNA were extracted from peripheral immune organs of Merino sheep and mRNA were purified to construct the cDNA library as template,according to the protocol of ZAP Express cDNA Synthesis Kit.Results showed that the cDNA library consisting of a primary contenet of 3.28×105 pfu and on amplified tite of 3.8×108 pfu/mL was constructed with the recombinant ratio of 97.7%.The average inserted fragments were about 1.5 kb with the majority ranging from 0.5 to 2.3 kb.It was concluded that the successfully constructed cDNA library has established a solid molecular basis for further study.

关 键 词：中国美利奴羊 外周免疫器官 CDNA文库 

分 类 号：S827.2[农业科学—畜牧学] Q959.842[农业科学—畜牧兽医]