棉花9-顺式环氧类胡萝卜素双加氧酶基因在干旱条件下的表达分析  

The Expression Analysis of Cotton 9-cis-epoxycarotenoid Dioxygenase Gene under Drought Stress

作　　者：刘江娜[1] 邓晓艳[1] 李志博[1] 刘菲菲[1] 章杰[1] 郗忠玲[1] 魏亦农[1] 

机构地区：[1]石河子大学农学院/新疆兵团绿洲生态农业重点实验室,石河子832003

出　　处：《石河子大学学报（自然科学版）》2010年第5期546-550,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30760124)

摘　　要：为了了解棉花幼苗9-顺式环氧类胡萝卜素双加氧酶(NCED)基因在干旱胁迫下的生物学功能,采用半定量逆转录聚合酶链式反应(semi RT-PCR)法检测在干旱胁迫下棉花GhNCED基因表达量的变化。结果表明:随着PEG6000介导的水分胁迫时间的推移,该基因的表达量逐渐上升,6 h时达到最强,随后又逐渐下降,到24 h时表达量降到最低。而在棉花不同的组织器官中,6 h时水分胁迫能显著促进棉花叶和茎中NCED基因的表达,根部GhNCED基因表达变化较小。In order to study the biologic function of 9-cis-epoxycarotenoid dioxygenase（NCED） gene under water-stress in cotton,a semi RT-PCR system was established to detect the gene expression of NCED in dehydrated cotton.The result showed that the expression level of the gene in leaves was gradually increased with the water-stressed accumulation,and it reached the highest level at the time point of 6 hours after PEG6000 and treatment and then it was gradually decreased to the lowest level after 24 hours＇ water stress.In different organs of cotton,the expression of GhNCED gene can be obviously induced at 6 hours after water stress treatment in stems and leaves,but the expression level of the gene in root was not obvious.

关 键 词：棉花 NCED 半定量RT-PCR 水分胁迫 

分 类 号：S513[农业科学—作物学] S718.43