玉米自交系8112特异SCAR标记的获得  

Conversion of RAPD Marker to SCAR Marker of Maize Inbred Line

作　　者：王斌[1] 张超良[1] 翁曼丽[1] 高红玉[1] 金德敏[1] 孙致良[2] 孙世孟[2] 

机构地区：[1]中国科学院遗传研究所 [2]莱阳农学院

出　　处：《高技术通讯》1999年第3期45-47,共3页Chinese High Technology Letters

基　　金：国家"九五"攻关资助项目

摘　　要：通过对３个玉米骨干自交系特异ＲＡＰＤ标记的克隆、测序、合成特异引物及ＳＣＡＲ－ＰＣＲ反应条件优化，成功地把其中一个自交系８１１２的特异ＲＡＰＤ标记（０．９ｋｂ）转换成了稳定的ＳＣＡＲ标记。Three unique RAPD markers of 3 maize elite inbred lines were cloned and sequenced separately. Pairs of SCAR markers were designed and synthesized according to the 5 terminal nucleotide sequences there. Optimizing SCAR PCR reaction conditions for each inbred line were carried out. Only the unique RAPD marker in inbred line 8112 was successfully conversed into the SCAR marker. It may provide a new way for the application of RAPD marker to germplasm identification and crop seed purity testing.

关 键 词：玉米自交系 种子 纯度检验 RAPD标记 SCAR标记 

分 类 号：S513.031[农业科学—作物学]