高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位  

Genetic analysis and mapping with SSR markers of the brown midrib gene bmr-6in Sorghum bicolor

作　　者：李杰勤[1] 王丽华[1] 詹秋文[1] 范军成[1] 

机构地区：[1]安徽科技学院植物科学学院,安徽凤阳233100

出　　处：《草业学报》2010年第5期273-277,共5页Acta Prataculturae Sinica

基　　金：科技部农业科技成果转化资金项目(No:2008GB2C300125);国家自然科学基金(No:31071470);安徽省"115"产业创新团队资助(No:皖人才办[2009]2号);国家科技支撑计划项目(No:2008BADB3B10);安徽省高校学科拔尖人才基金(No:教秘人[2005]79号);安徽科技学院稳定人才专项(No:ZRC2009236)资助

摘　　要：高粱棕色突变体的叶片中脉是棕色的,此类突变体能使家畜难以消化的木质素含量降低40%～60%,从而大幅度提高了家畜对高粱秸秆的消化率。利用高粱棕色中脉材料N592(bmr-6类型)和白色中脉(Sa)进行杂交和自交,构建F2分离群体,F1表现为白色中脉,F2白棕分离比符合3∶1,说明棕色中脉基因受一对等位基因控制,表现为隐性遗传。用已知定位到连锁群上的微卫星标记对棕色中脉基因进行了连锁分析,发现1个与棕色中脉(bmr-6)基因连锁的微卫星标记,连锁距离为4.2cM,初步将该基因定位于第7连锁群。The mutants,which produced brown leaf midribs,reduced the content of lignin by 40% to 60% compared with normal varieties.This low lignin content largely promoted the rate of digestion in livestock.The F2 population was constructed from the hybrid of N592（bmr-6）and Sa（white midrib）to map the bmr-6 gene. The ratio of white and brown midribs was 3to 1in the F2 population.Therefore,the bmr-6 gene is a single recessive gene.Using 171 SSR markers and F2recessive population,one SSR（simple sequence repeats）marker （txp295）that linked with the bmr-6gene was detected and the genetic distance was 4.2cM.In addition,the bmr-6gene was mapped to the seventh linkage group of sorghum because the txp295 marker was located on this linkage group.

关 键 词：高粱 bmr-6 SSR 基因定位 

分 类 号：S514[农业科学—作物学]