miRNA-124靶标功能的生物信息学挖掘  

Mining the Function of miRNA-124 Target by Bioinformatics Methods

作　　者：魏强[1] 李广林[1] 郭韬[1] 

机构地区：[1]陕西师范大学生命科学学院,中国陕西西安710062

出　　处：《生命科学研究》2010年第5期386-392,共7页Life Science Research

基　　金：国家自然科学基金资助项目(60970005);陕西省自然科学基金资助项目(2007F46);西安市科技攻关项目(NC09041);陕西师范大学校级优秀科技预研项目(200802010);陕西师范大学校级青年科技项目(200701004)

摘　　要：以miRNA-124为例,将miRNA的靶标预测信息与芯片数据分析中下调的基因信息整合起来,从而筛选出miRNA-124的候选靶标,然后对这些靶标的功能进行了深入分析.研究结果表明miRNA-124的靶标可分为两类:直接靶标和间接靶标,间接靶标是通过直接靶标的调控起作用的.miRNA-124靶标的功能主要是蛋白结合作用.miRNA-124靶标的筛选和功能挖掘,为科学工作者选择和验证其它miRNAs靶标的功能打下了坚实的基础,同时此方法可以推广到其它miRNAs的功能研究中.The miRNA-124 was taken as an example,and the miRNA-124 targets prediction information and the microarray data with the down-regulated gene information were integrated so as to screen the candidate of miRNA-124 targets.Thereby,function of the screened candidate was analyzed in depth.The research indicates that function of the miRNA-124 targets is binding action and there are two types of target：direct targets and indirect targets.Also,parts of the indirect targets take effect through regulation of the direct targets.Screening and mining function of the miRNA-124 targets lays a solid foundation for the scientists with selecting and verifying other type of functional miRNA targets.Simultaneously,the method can be extended to the study of other miRNA targets function.

关 键 词：miRNA-124 靶标 生物信息学 芯片分析 

分 类 号：Q74[生物学—分子生物学]