雪灵芝不同提取物的生物活性  

In Vitro Bioactivity of Different Extracts from Arenaria kansuensis Maxim

作　　者：刘笑笑[1] 万振江[1] 邓小波 石林[1] 卢晓霞[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]四川博力科技药业有限公司,成都610036

出　　处：《应用与环境生物学报》2010年第5期642-645,共4页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院知识创新工程项目资助~~

摘　　要：采用蛋白酪氨酸磷酸酯酶(PTP1B)、二肽基肽酶(DPP-IV)、α-葡萄糖苷酶-rat(α-glucosidase-rat)、MCF-7/ADR肿瘤耐药细胞增敏、肿瘤细胞毒活性(HepG2肝癌细胞)、骨髓有核细胞增殖等模型,对雪灵芝水提物、醇提物和市售的雪灵芝营养口服液分别进行体外免疫调节、抗肿瘤、糖尿病等活性筛选.结果表明:雪灵芝水提物、醇提物和营养口服液均对蛋白酪氨酸磷酸酯酶/PTP1B模型呈阳性,对其余5种模型呈阴性,说明蛋白酪氨酸磷酸酯酶(PTP1B)模型可以作为雪灵芝有效部位的活性跟踪模型.Biological activities of aqueous extract and ethanol extract from Arenaria kansuensis Maxim,as well as the commercial available oral nutritional liquid,were screened by a variety of in vitro models including proliferative effects on protein tyrosine phosphatase,dipeptidyl peptidase IV,inhibition of α-glucosidase,enhancement of sensitivity of drug-resistant MCF-7/ADR tumor cells,anti-tumor cytotoxic activities on HepG2 liver carcinoma cells and marrow cells were evaluated.The results indicate that all the samples tested exhibit inhibition on protein tyrosine phosphatase,whereas no obvious effects were observed on the other models.In conclusion,the active components of A.kansuensis can be traced by the protein tyrosine phosphatase model.

关 键 词：雪灵芝 水提物 醇提物 口服液 生物活性 

分 类 号：R285.5[医药卫生—中药学]