磷脂酶C参与ParA1诱导的烟草悬浮细胞死亡和防卫反应  

Involvement of Phospholipase C in Induction of Cell Death and Defense Responses in ParA1-Elicited Tobacco Suspension Cells

作　　者：魏芳芳[1] 王蕾[1] 王莲莲[1] 柳金伟[1] 梁元存[1] 王玉军[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《中国农业科学》2010年第20期4176-4182,共7页Scientia Agricultura Sinica

基　　金：山东省教育厅项目(J08LF06)

摘　　要：【目的】研究寄生疫霉Phytophthora parasitica分泌的蛋白激发子ParA1诱导烟草悬浮细胞后,磷脂酶C(phospholipase C,PLC)参与ParA1诱导烟草过敏细胞死亡(hypersensitive cell death,HCD)和防卫反应。【方法】采用药理学方法,在烟草悬浮细胞中添加PLC抑制剂,比较烟草悬浮细胞受ParA1诱导后细胞死亡和多种防卫反应的差别。【结果】PLC特异性抑制剂U73122能完全抑制ParA1引起的烟草悬浮细胞死亡,氧迸发、胞外基质碱化和植保素scopoletin(7-羟基-6-甲氧基香豆素)积累都能显著地被抑制,5种防卫基因hin1、hsr203J、PR(pathogenesis-related)-1a、PR-1b和PAL等的转录表达也都受到U73122不同程度的抑制。【结论】ParA1处理烟草悬浮细胞后,PLC参与了ParA1诱导细胞死亡和防卫反应的信号传导过程。【Objective】Phospholipase C（PLC） was investigated in response to ParA1,a protein elicitor from Phytophthora parasitica,and could trigger cell death and defense responses in tobacco suspension cells.【Method】Using a pharmacological approach,tobacco suspension cells pretreated with PLC inhibitors（neomycin and U73122） in ParA1-induced cells were used to compare cell death and a series of defense responses.【Result】Tobacco suspension cells treated with ParA1 resulted in defense responses,such as hypersensitive cell death,an oxidative burst,alkalization of the extracellular medium,expression of 5 defense-related genes and accumulation of scopoletin,defense-related genes hin1,hsr203J,PR-1a,PR-1b and PAL were expressed in ParA1-induced cells.Cell death induced by ParA1 was entirely inhibited by the addition of U73122,a PLC specific inhibitor. Oxidative burst,alkalization of the extracellular medium,expression of 5 defense-related genes and scopoletin accumulation was significantly suppressed by U73122,respectively.【Conclusion】PLC is required for ParA1-mediated cell death and defense response in tobacco suspension cells which may involve in signal transduction.

关 键 词：磷脂酶C ParA1 烟草 细胞死亡 防卫反应 

分 类 号：S432.2[农业科学—植物病理学]