浓缩六味地黄丸中马钱苷的药代动力学研究  

Pharmacokinetic of loganin in Liuweidihuang Concentrated Pill in Rat

作　　者：戴冰[1] 肖子曾[2] 梅君[2] 冷旺[2] 李兴丰[2] 赵婧[2] 廖建萍[1] 彭柳[2] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007

出　　处：《中成药》2010年第10期1692-1695,共4页Chinese Traditional Patent Medicine

基　　金：2008年省中医药局课题(2008099);2008年省科技厅课题(2008FJ3071)

摘　　要：目的:建立高效液相色谱法测定大鼠血清中马钱苷的浓度,研究大鼠灌胃给药后体内药代动力学特点。方法:采用KromasilC18色谱柱(4.6mm×250mm,5μm),流动相:0.05%磷酸水溶液(A)-乙腈(B),流速1.0mL/min,检测波长:236nm。结果:马钱苷回归方程:Y=1.0921X+27.064,r=0.9991,线性范围为26～420ng;平均回收率为94.20%,RSD=4.01%;样品24h内稳定,RSD=0.55%。大鼠灌胃后,药动学行为符合一室模型,主要药动学参数Ka、Ke、t1/2Ka、t1/2Ke、Tpeak、Cmax、AUC分别为0.0085/min、0.025/min、27.95min、81.23min、65.59min,6970.40ng/mL,1429620.62ng/(mL·min)。结论:首次建立了浓缩六味地黄丸中马钱苷血药浓度的HPLC测定方法,阐明了六味地黄丸中马钱苷的药代动力学参数,方法专属性强,结果可靠。AIM：To establish a revered-phase HPLC method for studying pharmacokinetics of loganin in serum of rat after perfusion.METHODS：The Kromasil C18（4.6 mm × 250 mm,5 μm）column was used as the analytical column and the mobile phase consisted of 0.05% phosphoric acid solution and acetonitrile,with the flow rate at 1.0 mL/min）.The UV detection was set at 236 nm.RESULTS：The calibration curves of loganin was linear in the range of 26 ～ 420 ng（r = 0.999 1）.The recovery and relative standard deviation of loganin was 94.20% and 4.01%,respectively.The loganin-containing serum was not changed in 24 hours（relative standard 0.55%）.After a perfusion of Liuweidihuang Concentrated Pill,the mean plasma concentrations-time relationship was found to fit a one-compartment open model,the main pharmacokinetic parameters of loganin were as follows：Ka,Ke,t1/2Ka,t1/2Ke,Tpeak,Cmax,AUC were 0.008 5 /min,0.025 /min,27.95 min,81.23 min,65.59 min,6 970.40 ng/mL,1 429 620.62 ng/（mL·min）.CONCLUSION：This is for the first time to establish the revered-phase HPLC method to determine concentrations of loganin in serum of Liuweidihuang Concentrated Pill and to obtain the pharmacokinetic parameters and characteristics.The method is reliable,the result is accurate.

关 键 词：浓缩六味地黄丸 马钱苷 药代动力学 

分 类 号：R969.2[医药卫生—药理学]