中药胃肠安丸中5种蒽醌类成分的定量分析  

Quantitative analysis of 5 kinds of anthraquinone in Weichang'an Pill

作　　者：张静泽[1] 高文远[1] 王磊[1,2] 马超一[1] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]天津中新药业集团股份有限公司乐仁堂制药厂,天津300380

出　　处：《中成药》2010年第10期1716-1720,共5页Chinese Traditional Patent Medicine

摘　　要：目的:建立中药胃肠安丸(木香、沉香、枳壳、檀香、大黄等)中蒽醌类成分的高效液相色谱含量测定方法。方法:色谱柱:Kromasil C18色谱柱(250mm×4.6mm,5μm);流动相:甲醇-0.5%醋酸溶液梯度洗脱;流速:1.0mL/min;检测波长:254nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚总含量在1.03mg/g以上,平均回收率为100.38%,所得结果的RSD值均小于2%。结论:该方法简便、灵敏、准确,为胃肠安丸的质量控制提供数据支持。AIM：To establish an HPLC method for determining free anthraquinones content in Weichang＇an Pill（Fructus Chaenomelis,Lignum Aquilariae resinatum,Fructus Aurantii,Lignum Santali albi,Radix et Rhizoma Rhei,etc.）.METHODS：The separation was performed on ODS column（250 mm × 4.6 mm,5 μm） of Kromasil.The mobile phase was a mixture of methanol-0.5% acetic acid solution,gradient elution,the flow rate was 1.0 mL/min and the wavelength was set at 254 nm.RESULTS：The total content of aloeemodin,rhein,emodin,chrysophanol and physcion was above 1.03 mg/g.The average recovery was 100.38%,RSD of the results was all less than 2%.CONCLUSION：The method is simple,accurate,sensitive and can be used to provide the data for the quality control of Weichang＇an Pill.

关 键 词：胃肠安丸 游离蒽醌 HPLC 

分 类 号：R927.2[医药卫生—药学]