高效催化3-氰基吡啶腈水解酶新菌株的筛选  

Screening of New Nitrilase-Producing Strains with High Activity of Hydrolyzing 3-Cyanopyridine

作　　者：杨春生[1] 金超[1] 周文瑜[1] 王学东[1] 魏东芝[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237

出　　处：《华东理工大学学报（自然科学版）》2010年第5期645-650,共6页Journal of East China University of Science and Technology

基　　金：国家973研究项目(2009CB724703)

摘　　要：通过富集培养技术,从土壤中筛选出1株具有较高3-氰基吡啶催化活力的产腈水解酶新微生物。实验主要通过让微生物在以甘油为碳源、腈类化合物为氮源的培养基中生长,达到筛选的目的。当以乙腈为氮源时,从土壤中筛选出具有芳基乙腈水解酶活力的菌株8株,其中2株表现出了对3-氰基吡啶的催化活力;当以3-氰基吡啶为氮源时,从土壤中筛选出具有芳香腈水解酶活力的菌株2株,它们对3-氰基吡啶具有较专一催化活力,其中活力最高的菌株被鉴定属于红细菌属(Rhodobacter),本文首次报道该种属菌株中具有腈水解酶。A new nitrilase producing strain showing high hydrolyzing activity towards 3-cyanopyridine was isolated from the soil using enrichment technique.Microorganisms were enriched with glycerol as the carbon source and nitriles as nitrogen source.Two different nitriles were used for screening,and it was found out and confirmed that 8 strains isolated dependent on acetonitrile as nitrogen source,can produce arylacetonitrilases,two of which showed 3-cyanopyridine hydrolysing activity;on the other hand,another two strains,compared with those former strains,show exclusive identification towards 3-cyanopyridine,as well as producing aromatic nitrilase when served with 3-cyanopyridine as nitrogen source.The one showing the higher activity towards 3-cyanopyridine is identified as Rhodobacter sp.,the capacity of which for producing nitrilase was first reported.

关 键 词：芳基乙腈水解酶 芳香腈水解酶 筛选 苯乙腈 3-氰基吡啶 

分 类 号：Q93[生物学—微生物学]