草鱼PGC-1α基因的表达及饲喂n-3 HUFAs对其影响  

PGC-1α gene expression and the influence of dietary n-3 HUFAs in grass carp ( Ctenopharyngodon idellus)

作　　者：吉红[1,2] 刘品[1] 李杰[1] 淡荣[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]西北农林科技大学水产科学研究所,陕西杨凌712100

出　　处：《水产学报》2010年第9期1327-1334,共8页Journal of Fisheries of China

基　　金：国家自然科学基金项目(30771667);西北农林科技大学青年学术骨干支持计划项目;中国博士后基金项目(20080431254)

摘　　要：获得了草鱼过氧化物酶体增殖物激活受体γ辅助活化因子1α(PGC-1α)部分cDNA序列(GenBank注册号为HM015283),并进行了序列同源性分析;采用实时定量反转录聚合酶链式反应(qRT-PCR)方法,检测了PGC-1α基因在草鱼不同组织的表达状况;研究了投喂高不饱和脂肪酸(HUFAs)对草鱼肝胰脏PGC-1α基因时序表达的影响。结果显示,所获得的草鱼PGC-1α基因部分cDNA序列长度为612bp,与人、牛、小鼠、斑马鱼和金鱼等动物的同源性为75%～97%;该基因在草鱼肝胰脏、肾脏、肌肉、心脏、鳃等10个组织中均有表达,其中在肾脏和心脏中表达丰度最高,在精巢和脾脏中表达丰度最低;草鱼摄食HUFAs饲料后第7天PGC-1α基因的表达水平极显著升高,随后又迅速下降。研究首次克隆得到草鱼PGC-1α基因部分cDNA序列,并发现该基因在草鱼能量代谢水平高的组织中表达较高,且其表达受到HUFAs的调控,其时序表达模式为先升高,然后恢复到正常水平。The partial cDNA of peroxisome proliferator activated receptor-gamma coactivator-1 alpha（ PGC- 1α） was cloned（ GenBank accession number HM015283） ,and the homologous alignment was analyzed. The expression in different organs of PGC-1α was detected in grass carp with the methods of qRT-PCR. The influence of dietary highly unsaturated fatty acids （ HUFAs） on the temporal expression of PGC-1α in hepatopancreas of grass carp was also studied. The results showed that the obtained cDNA sequence was 612 bp in length,and its homology with human,cow,mice,zebra fish and other species is 75% -97% ; and it is revealed that PGC-1α is widely expressed in hepatopancreas,kidney,muscle,heart,gill,and so on,with the highest expression level observed in kidney and heart,lowest in spermary and spleen. After six days feeding with HUFAs contained diet,the expression level of PGC-1α was significantly increased,then descended quickly in the following days. Our results indicate that PGC-1α is mainly express in high energy generating tissues and its expression level described as could be regulated by HUFAs. The expression pattern of PGC-1α induced by dietary HUFAs could be rising at first then going down back to the normal level.

关 键 词：草鱼 PGC-1Α 基因表达 高不饱和脂肪酸 

分 类 号：S917[农业科学—水产科学]