南海北部海域多齿蛇鲻的种群分析  

Population analysis of Saurida tumbil in the Northern South China Sea

作　　者：孙冬芳[1,2] 董丽娜[1,2] 李永振[1] 卢伟华 李希国 于杰[1] 黄梓荣[1] 艾红[1] 朱文聪[1] 崔科[2] 李娜娜[4] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]东莞市海洋与渔业环境监测站,广东东莞523079 [4]大连海洋大学,辽宁大连116023

出　　处：《水产学报》2010年第9期1387-1394,共8页Journal of Fisheries of China

基　　金：科技部社会公益研究专项资金项目(2004DIB3J098);广东省科技计划项目(2005B31001005);国家科技基础性工作重点项目(2003DEA6N042);国家农业财政项目(2009-2011)

摘　　要：对南海北部海域5个多齿蛇鲻地理群体(北海、湛江、茂名、东莞、汕尾)共78个个体的线粒体控制区基因序列进行了扩增测序。在78个个体的568bp序列中共检测到48个单倍型,39个变异位点。经分子变异等级分析(AMOVA)得出5个地理群体内遗传变异度为98.82%,群体间遗传变异度仅为1.18%,各群体间成对遗传变异固定指数FST值在0.00024～0.03008;78个个体间的遗传距离在0～0.025。通过对基于序列片段构建的NJ树和基于序列单倍型利用中介网络法构建的群体间关系网的分析发现,5个群体间有明显的基因交流,遗传分化差异不大;再结合种群、种及属的界定标准,判定5个多齿蛇鲻群体归属于同一种群;而且,在经历快速增长、变异和瓶颈期之后,多齿蛇鲻种群就以小群体模式继续快速增长,形成地理群体。Since fish population is a basic unit of study and management of fishery resources,analysis of fish population is a foundation for biological and ecological study on fish reproduction,distribution and migration. Using molecular biology method,the paper analyzed population of Saurida tumbil,an important commercial species from northern continental shelf waters of South China Sea. A total of 78 samples of Saurida tumbil were collected from Beihai,Zhanjiang,Maoming,Dongguan,and Shanwei of northern South China Sea. We found there are 48 haplotypes and 39 variation sites in 568 bp gene fragments of 78 sequences obtained by sequencing the mitochondrial D-loop of the samples. Analysis of molecular variance（ AMOVA） indicates that the genetic variability within groups is 98. 82% and that among groups is only 1. 18% . The genetic differential index（ Fst） and the genetic distance between every two groups are 0. 000 24 - 0. 030 08 and 0 -0. 025,respectively. Molecular tree constructed by NJ method and the network constructed by TCS1. 21 show that the individuals of different groups are not clustered as one independent group and intermixed together,which indicates that there clearly exists a great gene flow among groups,and the genetic diversities among groups are at quite lower levels. According to the criteria distinguishing different populations,species and genera given by scholars Theorpe and Shaklle,the five groups should be merged to the same population. However,Saurida tumbil population built its geological groups by means of continuously rapid growth in small ones after undergoing fast expansion,differentiation and bottleneck phase,which not only accords with the traditional viewpoint of basic characteristics of fishery resources of South China Sea to a certain extent— ＂The populations of most species belong to local population without a long-distance migration＂,but also is regarded as an evidence of molecular biology.

关 键 词：多齿蛇鲻 种群分析 遗传结构 南海 

分 类 号：S931.5[农业科学—渔业资源]