狼疮鼠骨髓树突状细胞的分离培养和免疫学特性  

Isolation,Culture and Immunobiological Characteristics of Dendritic Cells from Lupus Mouse Bone Marrow

作　　者：毛越苹[1] 齐庆[2] 罗益金[1] 谭国珍[1] 曾凡钦[1] 

机构地区：[1]中山大学附属第二医院皮肤性病科,广州510120 [2]广州中医药大学附属第一医院皮肤性病科,广州510405

出　　处：《热带医学杂志》2010年第9期1039-1041,共3页Journal of Tropical Medicine

基　　金：广东省科技计划项目(No.2007B031506002);广东省中医药局科研项目(No.2007105)

摘　　要：目的对狼疮鼠骨髓树突状细胞进行分离、培养并分析其免疫学特性,为进一步研究和应用狼疮鼠骨髓来源树突状细胞(DC)奠定基础。方法分离、纯化6周龄雄性BXSB狼疮鼠骨髓单核细胞,以含10%胎牛血清、2ng/ml重组小鼠粒细胞-巨噬细胞集落刺激因子(GM-CSF)和20ng/ml重组小鼠白细胞介素-4的RPMI-1640培养基培养,以脂多糖(LPS,1μg/ml)刺激24h体外诱导BXSB狼疮鼠骨髓细胞分化为DC,倒置显微镜动态观察细胞形态学变化,流式细胞仪分析细胞表面分子,混合淋巴细胞反应检测其刺激T细胞增殖能力。结果经体外诱导培养第2～8天可见大量细胞集落形成,培养的DC具有典型树突状形态,同时DC可显著刺激同种异体混合淋巴细胞增殖。结论体外诱导培养可稳定获得BXSB狼疮鼠骨髓来源DC。Objective To isolate, culture the myeloid dendritic cells （DC） of lupus mouse, and to investigate their immunobiological characteristics.Methods BXSB lupus mouse bone marrow cells were induced by 2 ng/ml recombinant mouse-granulocyte and macrophage colony-stimulus factor（GM-CSF）, 20 ng/ml interleukin-4 （IL-4） and 1 μg/ml lipopolysaccharide （LPS） in vitro.The morphological characteristics of DC were observed with light inverted microscope.Cell surface molecules were identified by flow cytometry.The immunobiological function was studied with mixed lymphocyte reaction （MLR）.Results The cultured cells displayed typical morphological characteristics of DC, and could be stimulated by the mixed lymphocyte.Conclusion In vitro, lupus mouse myeloid DC can be induced and cultured.

关 键 词：狼疮鼠 树突状细胞 培养 

分 类 号：R446.61[医药卫生—诊断学]