等离子体磺酸化丝素蛋白膜聚四氟乙烯复合小口径人工血管体外的实验研究  

Hemocompatibility evaluation in vitro of small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma

作　　者：李少彬[1] 闫玉生[1] 李辉[1] 陈坤棠[1] 童健[1] 陈群清[1] 张福伟[1] 肖飞[1] 

机构地区：[1]南方医科大学珠江医院胸心外科,广东广州510282

出　　处：《南方医科大学学报》2010年第9期2100-2103,共4页Journal of Southern Medical University

基　　金：广东省医学科学技术研究基金(WSTJJ20071104410303197905132512);广东省科技计划项目(93025)

摘　　要：目的研究等离子体磺酸化丝素蛋白膜聚四氟乙烯复合小口径人工血管(复合血管)的抗凝血性能,并探讨其安全性、可行性。方法采用低温等离子体技术将涂覆有丝素蛋白膜的聚四氟乙烯小口径人工血管磺酸化,制成复合血管,行体外溶血实验检测复合血管的溶血相关安全性,同时行血小板粘附、动态凝血、复钙实验评价复合血管血液相容性。结果扫描电镜照片看到,普通人工血管表面有血小板粘附,且血小板完全展开,呈扁平状紧贴在材料表面上,复合血管组表面极少血板粘附;复合血管组吸光度-时间变化曲线位于普通血管之上,且下降趋势较缓,经历时间较长,与普通血管相比,其抗凝血性能较好;复钙实验中复合血管组平均时间为480s,小于对照组平均时间603s,两样本t检验提示P=0.000,提示两者有显著统计学差异。结论等离子体磺酸化丝素蛋白膜聚四氟乙烯复合小口径人工血管具有良好的溶血安全性及抗凝血性能。Objective To evaluate the hemocompatibility of a small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma treatment.Methods The composite blood vessel was prepared by first coating the small-caliberexpanded polytetrafluoroethylene vessel with silk fibroin followed by sulfonation by low temperature plasma treatment.Afterhemolysis test in vitro,dynamic coagulation time test,blood platelet adhesion test,and recalcification time test were performed to evaluate the hemocompatibility of the composite blood vessel.Results Scanning electronic microscopy revealed obvious platelets adhesion on the conventional artificial（control） vessel,which seldom occurred on the composite vessel.The curve of absorbance-clotting time of the composite vessel declined more slowly than that of the control vessel.The recalcification time of the composite blood vessel averaged 603 s,significantly longer than that of the control vessel（480 s,P=0.000）.Conclusion The composite blood vessel has good antithrombotic activity and hemocompatibility as a promising vascular prosthesis.

关 键 词：人工血管聚四氟乙烯丝素蛋白低温等离子体血液相容性 

分 类 号：R318.1[医药卫生—生物医学工程]