肾交感神经在哇巴因高血压鼠肾脏钠转运中的作用  

Role of renal sympathetic nerves in renal sodium transport in ouabain-hypertensive rats

作　　者：葛蘅[1] 姜馨[2] 任延平[3] 吕卓人[3] 

机构地区：[1]西安交通大学医学院第二附属医院肾内科,陕西西安710004 [2]陕西省人民医院老年心血管病科,陕西西安710068 [3]西安交通大学医学院第一附属医院老年心血管病科,陕西西安710061

出　　处：《南方医科大学学报》2010年第9期2111-2114,2118,共5页Journal of Southern Medical University

基　　金：陕西省自然科学基金(2004C213)

摘　　要：目的哇巴因可以使Sprague-Dawley(SD)大鼠肾脏近端小管钠重吸收增加,在高血压的发生和发展中起着重要的作用。本研究探讨肾交感神经在哇巴因高血压鼠(OHR)肾脏钠转运中的作用。方法将双侧去肾交感神经(RDNX)的SD大鼠及假手术鼠分别随机分入对照组和哇巴因组。对照组每日给予腹腔注射生理盐水1ml/(kg·d),哇巴因组每日给予腹腔注射哇巴因27.8μg/(kg·d),每周测量收缩压(SBP)、体质量,心率各1次。实验过程中各组动物的饲养及生活条件完全相同。于治疗4周后处死。处死前1d用代谢笼收集24h尿,禁食过夜,次日晨取血后处死。肾脏称重后,置于-70℃冰箱保存。血清及尿样标本置于-40℃冰箱保存,同批测定。采用火焰原子吸收分光光度法检测血,尿钠浓度,原子吸收分光光度法测定血,尿内源性锂浓度;苦味酸法测定血,尿肌酐浓度;放射免疫法测定血浆肾素活性;高效液相-电化学法测定肾脏去甲肾上腺素含量。结果四组大鼠体质量、心率、摄食量及钠摄入量均无显著性差异(P>0.05)。治疗4周后,对照去神经组(CDNX)SBP显著低于对照假手术组(Csham),哇巴因去神经组(ODNX)SBP亦显著低于哇巴因假手术组(Osham)(P均<0.05),且SBP同样下降约10mmHg。而Osham组及ODNX组血压显著高于相应的对照组(P均<0.01)。ODNX组血压亦显著高于Csham(P<0.01)。哇巴因组的钠排泄分数(FENa),锂排泄分数(FELi)及远端肾小管钠重吸收率(FDRNa)分别显著低于相同处理的对照组(P<0.05,P<0.01,P<0.05),说明哇巴因组无论是否去肾神经,近端小管钠重吸收与对照组相比都显著增加,肾脏钠排泄及远端小管钠重吸收均显著减少;而ODNX组与Osham组相比,FENa,FELi及FDRNa均无显著性差异(P>0.05)。CDNX组与Csham组相比,FENa,FELi及FDRNa亦无显著性差异(P>0.05),表明去肾神经与否,对肾钠转运功能没有影响。各组间内生肌酐清除率(Ccr)及血浆肾素活性无显�Objective To investigate the role of renal sympathetic nerves in renal sodium transport in ouabain-hypertensive rats（OHR） .Methods Sixteen male SD rats with sham renal denervation（Sham-RDNX） and 16 with renal denervation（RDNX） were randomly into normal control group and ouabain group to receive intraperitoneal injection of normal saline（1 ml/kg） and ouabain（27.8μg/kg） once a day,respectively.Systolic blood pressure（SBP）,heart rate and body weight were recorded weekly.Food consumption of the rats was determined twice a week.After a 4-week treatment,blood and 24 h urine samples were collected to measure the serum and urinary concentration of sodium,trace lithium and creatinine.Endogenous creatinine clearance rate（Ccr）,fractional excretions of sodium（FENa）,fractional excretions of lithium（FELi） and fractional reabsorption of sodium in the postproximal tubules（FDRNa） were calculated.Plasma renin activity was determined by radioimmunoassay.Norepinephrine was extracted from the renal tissue and assayed for norepinephrine content by HPLC.Results The body weight,food intake and heart rate showed no significant difference among the 4 groups（P〉0.05） .After 4 weeks,the SBP of control RDNX group（CDNX） was significantly lower than that of the control Sham-DNX group（Csham）（P〈0.05）;the SBP of ouabain RDNX group（ODNX） was also significantly lower than that of ouabain Sham-DNX group（Osham）（P〈0.05）;RNDX lowered SBP by about 10 mmHg in both ouabain groups and control groups.The SBP was significantly higher in Osham and ODNX groups than in the corresponding control groups（P〈0.01）,also significantly higherin ODNX group than in Csham group（P〈0.01） .Ccr showed no significant difference among the 4 groups（P〉0.05） .FENa,FELi and FDRNa were significantly lower in ouabain groups than in the corresponding control groups（P〈0.05,P〈0.01,P〈0.05）,but FENa,FELi and FDRNa of ODNX group were similar with those of Osham group（P〉0.05）;FENa,FEL

关 键 词：哇巴因 高血压 肾去神经 肾脏钠转运 

分 类 号：R544.1[医药卫生—心血管疾病]