整体和离体照射致大鼠淋巴细胞T细胞受体基因突变的一致性研究  

Consistency of T-cell receptor gene mutation between lymphocytes in rats after irradiation in vivo and in vitro

作　　者：石燕[1] 刘伟[1] 侯殿俊[1] 马娅[1] 乔建维[1] 卢峰[1] 商希梅[1] 

机构地区：[1]山东省医学科学院放射医学研究所,济南250062

出　　处：《中华放射医学与防护杂志》2010年第5期561-563,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：山东省自然科学基金（Y2007C151）

摘　　要：目的 探讨整体和离体照射所致的大鼠淋巴细胞T细胞受体（TCR）基因突变的一致性.方法 48只雌性大鼠随机分为6组,每组8只,分别取外周静脉血标本,经0、0.5、0.75、1.0、2.0和3.0 Gy照射.用相同方法 照射各组大鼠,照后取外周血标本.所有淋巴细胞体外培养7 d后,用单克隆抗体直接免疫荧光标记流式细胞术检测TCR突变频率（TCRMF）.结果 整体和离体照射组TCR基因突变频率均呈剂量依赖性增高,两组间TCRMF比较,差异无统计学意义（t=-1.1～0.3,P＞0.05）.结论 整体和离体照射所致的大鼠淋巴细胞TCR基因突变频率一致,即外周血淋巴细胞经X射线照射体外培养后,流式细胞术检测,可客观准确的反映全身照射的情况.Objective To observe the consistency of T-cell receptor （TCR） genes mutation in lymphocytes in rats after irradiation in vivo and in vitro.Methods A total of 48 female rats were randomly divided into 6 equal groups.Peripheral blood samples from them were collected to separate the lymphocytes and then irradiated to X-ray irradiation with the dose rate of 200 cGy/min at the doses of 0,0.5,0.75,1.0,2.0,and 3.0 Gy,respectively.Then all the lymphocyte samples were cultured for 7 days.Flow cytometry with direct immunofluorescence was used to detect the TCR gene mutation.The levels of TCR gene mutant frequency （TCRMF） of different groups were calculated.Results The TCRMF levels of different groups after irradiation in vivo and in vitro all displayed a dose-dependent manner and there were no significant differences in the TCRMF between different dose irradiation groups（t = -1.1-0.3 ,P ＞0.05）.Conclusions A consistency of TCRMF after irradiation in vivo and in vitro is proven.The results of TCRMF of peripheral blood lymphocytes irradiated in vitro by flow cytometry can precisely reflect the TCR genes mutation after whole-body irradiation.

关 键 词：T细胞受体基因 生物剂量计 整体照射 离体照射 

分 类 号：R818.02[医药卫生—放射医学]