N-甲基-D-天冬氨酸受体NR1亚基调控大脑皮层神经元放射状迁移  

NR1 Subunit of N-methy-D-asparate receptor regulates the radial migration of cortical neurons

作　　者：王莹[1] 

机构地区：[1]温州医学院检验医学院生命科学学院,浙江温州325035

出　　处：《解剖学报》2010年第5期674-678,共5页Acta Anatomica Sinica

基　　金：温州医学院科研启动基金(QTJ09013)资助

摘　　要：目的探讨N-甲基-D-天冬氨酸(NMDA)受体在大脑皮层发育过程中参与调控皮层神经元放射状迁移的情况。方法通过在体子宫内胚胎电转基因和RNA干扰技术(RNAi),在发育的大鼠胚胎脑内干扰NMDA受体的NR1亚基表达,结合荧光免疫组织化学技术,追踪基因转染上的神经元在大脑发育过程中的迁移途径和特征。结果转染上对照质粒的皮层神经元能通过放射状迁移,在出生后第7天(P7)之前迁移到皮层的正确位置,即2～3层,而干扰NR1基因表达后的神经元则不能正常地放射状迁移至皮层的2～3层,P7时仍停留在皮层深层位置,甚至发生区。结论 NMDA受体在大脑皮层神经元放射状迁移过程中起关键性作用,有助于理解胚胎发育过程中迁移的机制并为预防控制神经元迁移异常提供依据。Objective To investigate whether and how N-methy-D-asparate （NMDA） receptor regulates radial migration of cortical neuron. Methods Migration route and characters of transefected neurons were traced, using in utero electroporation of small interference RNA （siRNA） of NMDA receptor NR1 subunit, the essential subnnit, and fluorescence immunohistochemistry. Results Cortical neurons transfected with control vector,could radially migrate to the proper cortical plate layer 2-3 before postnatal 7 （P7） , however, neurons which silenced NR1 subunit could not migrate to the proper layer 2-3, while improperly stayed at lower layers or even the germinal regions. Conclusion NMDA receptor plays an essential role in regulating proper neuronal migraion and cortical lamination in the developing rat cortex, which is helpful to understand the basic mechanism of neuron migration and prevent neuronal migration disorders.

关 键 词：神经元 放射状迁移 N-甲基-D-天冬氨酸受体 在体子宫内胚胎电转基因 免疫荧光组织化学 大鼠 

分 类 号：R338[医药卫生—人体生理学]