肝细胞癌患者外周血Th17细胞水平的变化及其与肿瘤进展的关系  

Increased level of Thl7 cells in peripheral blood correlates with the development of hepatocellular carcinoma

作　　者：王维维[1] 王振猛[2] 刘耀阳[1] 秦阳华[1] 沈茜[1] 

机构地区：[1]第二军医大学附属长海医院实验诊断科,上海200433 [2]第二军医大学附属东方肝胆医院麻醉科

出　　处：《中华肿瘤杂志》2010年第10期757-761,共5页Chinese Journal of Oncology

摘　　要：目的 研究Th17细胞在肝细胞癌（HCC）外周血中的表达,探讨Th17与HCC进展的关系.方法 采用流式细胞术检测61例HCC患者和38例正常对照者外周血中Th17细胞的水平,应用实时荧光定量聚合酶链反应（QRT-PCR）,从mRNA水平检测白细胞介素（IL-17）、IL-23p19和维甲酸相关孤儿受体（RORc）的表达情况,分析HCC患者外周血Th17水平变化与其各项临床指标间的关系.结果 61例HCC患者的Th17细胞水平为4.67%±0.79%,正常对照组为3.25%±0.68%,两组间差异有统计学意义（P＜0.0001）.HCC患者外周血单个核细胞（PBMC）中IL-17、IL-23p19和RORc的mRNA表达水平分别为正常对照组的23.9、8.5和23.2倍,差异有统计学意义（P＜0.05）.HCC患者外周血中Th17水平增高与患者的性别、年龄、术前甲胎蛋白浓度等无关（P＞0.05）,而与肿瘤大小、门脉癌栓、远处转移、TNM分期等有相关性（P＜0.05）.Th17细胞水平随TNM分期的增加而升高,Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期患者外周血中Th17细胞水平分别为4.05%±0.82%、4.32%±0.67%、4.94%±0.70%和5.22%±0.87%,Ⅰ～Ⅱ期患者的Th17细胞水平明显低于Ⅲ～Ⅳ期（P=0.0008）.结论 HCC患者外周血中Th17细胞水平升高,Th17细胞水平增高与肿瘤大小、门脉癌栓、远处转移、TNM分期有明显关系;PBMC中IL-17、IL-23p19和RORc的mRNA表达水平也明显升高.Th17细胞可能通过分泌效应细胞因子,直接或间接参与了HCC细胞的增殖、侵袭和远处转移.Objective To investigate the potential correlation between the level of Th17 cells in peripheral blood and the development of human hepatocellular carcinoma （HCC）. Methods Th17 cells in the blood samples from 61 HCC patients and 38 healthy controls were assessed by flow cytometry （ FCM ）.The mRNA expression levels of IL-17, IL-23p19 and RORc in peripheral blood mononuclear cells （PBMC）were detected by quantitative real-time PCR. The potential correlation of increased Th17 cells in blood with the clinical characteristics of the 61 patients, including gender, age, preoperative AFP concentration, tumor diameter, portal vein tumor thrombus （ PVTT）, metas tasis and TNM stages was analyzed. Statistical analysis was performed using the software GraphPad Prizm 5.0. Results The number of Th17 cells in 61 HCC patients was significantly higher than that in the normal controls （4.67% ±0.79% vs 3.25% ±0.68%,P〈0.0001）. The same tendency was also found in the mRNA levels of IL-17, IL-23p19 and RORc in PBMC （P 〈0.05 ）. The increased level of Th17 cells in HCC patients showed a positive correlation with the tumor size, PVTT, metastasis and TNM stages （P〈0.05 for each group）. The level of Th17 cells in HCC patients was increased along with the increasing TNM stages Ⅰ to stage Ⅳ： 4.05% ±0.82% ,4.32% ±0.67% ,4.94% ±0.70% ,and 5.22% ±0.87%, resprctively, where the level of Th17 cells in patients with advanced stage of HCC （ Ⅲ - Ⅳ ） was significantly higher than that in early stage （ Ⅰ - Ⅱ , P =0.0008）. Conclusion The increased of level of Th17 cells in peripheral blood of HCC is significantly correlated with the tumor size, PVTT, metastasis and TNM stage, indicating that the Th17 cells might participate in promoting invasion and progression of HCC directly or indirectly by secreting characteristic cytokines.

关 键 词：癌 肝细胞 TH17细胞 白细胞介素17 维甲酸相关孤儿受体 增殖 侵袭 转移 

分 类 号：R686[医药卫生—骨科学]