骨髓间充质干细胞移植对吸入性损伤家兔炎症反应和肺损伤的影响  

Effect of bone marrow-derived mesenchymal stem cells transplantation on the inflammatory response and lung injury in rabbit with inhalation injury

作　　者：朱峰[1] 郭光华[2] 陈雯[2] 彭燕[2] 邢娟娟[2] 王年云[2] 

机构地区：[1]南昌大学第一附属医院重症医学科,330006 [2]南昌大学第一附属医院烧伤科,330006

出　　处：《中华烧伤杂志》2010年第5期360-365,共6页Chinese Journal of Burns

基　　金：江西省教育厅青年科学基金（GJJ09432）

摘　　要：目的 初步了解骨髓间充质干细胞（MSC）移植对烟雾吸入性损伤兔外周血主要炎症因子和肺水质量分数以及肺组织损伤的影响. 方法 取16只成年新西兰大耳白兔制成烟雾吸入性损伤模型后,按随机数字表法分成单纯致伤组和MSC移植组（各8只）.单纯致伤组伤后立即经耳缘静脉注入10 mL PBS;MSC移植组伤后立即经耳缘静脉注入10 mL PBS,内含兔第3代MSC（分离自健康幼龄新西兰大耳白兔）1×107个.另取8只成年新西兰大耳白兔作为正常对照组,不致伤,仅经耳缘静脉注入10 mL PBS.单纯致伤组和MSC移植组分别于致伤后2、4、6 h采血,以ELISA法检测血清TNF-α、IL-1β、IL-6、IL-10含量;伤后24 h,取兔肺行大体观察和组织病理学观察（左下肺）,取右肺中叶组织计算肺水质量分数.正常对照组同法抽血并取肺组织进行检测.对实验数据行t检验.结果 （1）两致伤组家兔各时相点血清TNF-α含量均明显高于正常对照组（t=2.43～9.57,P＜0.05或P＜0.01）.单纯致伤组各时相点血清IL-1β和IL-6含量明显高于正常对照组（t=8.49～19.80,P值均小于0.01）;MSC移植组各时相点IL-1β含量与正常对照组接近（t=0.11～0.92,P值均大于0.05）,IL-6含量伤后2 h与正常对照组接近（t=2.12,P＞0.05）,4、6 h显著升高（t值均为2.83,P值均小于0.05）.MSC移植组家兔各时相点血清TNF-α、IL-1β、IL-6明显低于单纯致伤组（t=2.35～12.45,P＜0.05或P＜0.01）.（2）MSC移植组伤后2、4、6 h血清IL-10含量分别为（13.0±3.6）、（11.6±8.5）、（15.2±4.4）pg/mL,与单纯致伤组各对应时相点的含量[分别为（5.5±3.4）、（5.0±1.7）、（7.9±3.5）pg/mL]相比均显著升高（t值分别为4.28、2.15、3.67,P值均小于0.01）.两致伤组亦明显高于正常对照组（t=2.46～8.14,P＜0.05或P＜0.01）.（3）大体观察及组织病理学观察见,MSC移植组肺组织损伤程度较单纯致伤组明显改善.（4）伤后24 hObjective To study the effect of bone marrow-derived mesenchymal stem cells （MSC） transplantation on the major inflammatory cytokines content in peripheral blood, lung water mass fraction, and lung tissue injury in rabbits with smoke inhalation injury. Methods Sixteen adult New Zealand big ear rabbits were subjected to smoke inhalation injury and then were divided into pure injury group （PI, n =8） and MSC transplantation group （ MT, n = 8） according to the random number table. Via ear marginal vein, rabbits in PI group were injected with 10 mL phosphate buffered saline （PBS）; rabbits in MT group were injected with 10 mL PBS containing the third passage MSC （ 1 × 107 cell） isolated from marrow of healthy young New Zealand big ear rabbit. Another 8 rabbits were enrolled as normal control group （ NC ）. Rabbits in NC group were injected with 10 mL PBS via ear marginal vein without smoke inhalation injury. Blood was harvested from rabbits in PI and MT groups at post injury hour （PIH） 2, 4, 6. Contents of TNF-α, IL-1β, IL-6, and IL-10 in serum were determined with ELISA. At PIH 24, left lung was harvested for morphology and histopathology observation; the right lung tissue was obtained to measure and calculate lung water mass fraction. Blood and lung tissue of rabbits in NC group were harvested and determined in the same way. Data were processed with t test. Results （ 1 ） Serum contents of TNF-α in PI and MT groups at each time point were obviously higher than that in NC group （ t =2.43-9.57, P ＜0.05 or P ＜0.01 ）. Serum contents of IL-1β and IL-6 in PI group at each time point were obviously higher than those in NC group （ t = 8.49-19.80, P values all below 0.01 ）. Serum content of IL-1 β in MT group at each time point was close to that in NC group （ t = 0.11-0.92, P values all above 0.05）. Serum content of IL-6 in MT group at PIH 2 was close to that in NC group （ t =2.12, P ＞0.05）, but that of MT group increased significantly at PIH 4 and 6 （ t = 2.83, P va

关 键 词：烧伤 吸入性 骨髓间充质干细胞 炎症因子 肺损伤 肺水质量分数 

分 类 号：R686[医药卫生—骨科学]