IL-22参与介导NOD小鼠自发性胚胎丢失  

Relationship between IL-22 expression and the spontaneous embryo loss in NOD mice

作　　者：许洁涵[1] 陈琳琳[2] 赵晓明[1] 陈翃[3] 王文静[2] 林羿[3] 

机构地区：[1]上海交通大学医学院附属仁济医院生殖医学中心,上海200001 [2]暨南大学组织移植与免疫研究中心,广州510632 [3]上海交通大学医学院附属仁济医院妇产科学研究所,上海200001

出　　处：《现代免疫学》2010年第5期370-374,共5页Current Immunology

基　　金：国家自然科学基金资助项目(30872761)

摘　　要：为研究IL-22与NOD小鼠自发性胚胎吸收率增高的关系,多次腹腔注射中和抗体抑制IL-22,采用流式细胞术检测子宫CD4+IL-22+细胞百分率,并观察NOD×C57BL/6小鼠交配组合中孕鼠胚胎吸收率的变化。结果显示,NOD×C57BL/6孕鼠子宫CD4+IL-22+细胞百分率显著高于BALB/c×C57BL/6孕鼠(52.4%±2.1%与14.3%±4.4%,P<0.01)。多次腹腔注射中和抗体可显著降低NOD×C57BL/6孕鼠子宫内CD4+IL-22+细胞数量(18.7%±2.8%;与未抑制组相比,P<0.01),并可显著降低这些小鼠孕12.5 d的胚胎吸收率(4.8%±2.0%与19.1%±5.1%,P<0.05)。线性相关-回归分析显示,胚胎吸收率与子宫CD4+IL-22+细胞百分率呈正相关。提示妊娠子宫内CD4+IL-22+细胞相对数量过多是妊娠不利因素,可能是造成NOD×C57BL/6孕鼠胚胎吸收率增高的原因之一。To investigate the relationship between IL-22 expression and the increase of embryo resorption rate in NOD mice.multiple peritoneal injection of neutralizing antibody against IL-22 was performed in female NOD mice impregnated by C57BL/6 males.The percentage of CD4＋IL-22＋ cells within CD4＋ cell population was detected by using two-color flow cytometry and the fetal resorption rate was measured on day 12.5 of gestation.It was demonstrated that the percentage of uterine CD4＋IL-22＋ cells within CD4＋ population was significantly higher in NOD C57BL/6 matings than that of BALB/c C57BL/6 matings（52.4±2.1% vs 14.3±4.4%,P0.01）.Multiple administrations of anti-mouse IL-22 significantly decreased the number of uterine CD4＋IL-22＋ cells（18.7±2.8%;P0.01 versus no-inhibition group）,and significantly decreased the embryo resorption rate on day 12.5 in these mice（4.8±2.0% vs 19.1± 5.1%,P0.05）.Linear regression assay showed that the fetal resorption rate was positively correlated with the percentage of CD4＋IL-22＋ cells within CD4＋ population.It suggests that over-expression of IL-22 in uterine CD4＋ cells may be harmful to the pregnancy outcomes,and may be one of the reasons causing the increase of the fetal resorption rate in NOD×C57BL/6 matings of mice.

关 键 词：流产 母-胎界面 免疫调节 免疫耐受 

分 类 号：R392.11[医药卫生—免疫学]