脂多糖对心肌细胞缺氧诱导因子-1α表达的影响  

Effects of Lipopolysaccharide on the expression of hypoxia-inducible factor-1α in cardiomyocyte

作　　者：高翔[1] 毛奕[2] 邹晓静[3] 杨乐[4] 

机构地区：[1]华中科技大学同济医院综合科,武汉430030 [2]华中科技大学协和医院心内科 [3]华中科技大学协和医院麻醉科 [4]华中科技大学同济医院急诊内科

出　　处：《临床心血管病杂志》2010年第8期629-631,共3页Journal of Clinical Cardiology

摘　　要：目的:观察脂多糖(LPS)对心肌细胞损伤及缺氧诱导因子-1α(HIF-1α)表达的影响。方法:培养新生乳鼠心肌细胞,以LPS(1μg/ml)分别作用0～4h,建立脓毒症心肌抑制细胞模型,测定各时点细胞存活率、LDH漏出率、锥虫蓝摄取率及肌酸激酶(CK)活性。用免疫印迹法测定LPS对HIF-1α表达的影响。结果:LPS处理后,LDH漏出率、锥虫蓝摄取率及CK活性均明显上调,而细胞存活率下调,同时伴HIF-1α表达明显上调。结论:LPS能导致明显的心肌损伤;同时伴随HIF-1α表达明显上调,但HIF-1α在脓毒症心肌抑制中扮演的角色尚需进一步研究。Objective：To observe effects of LPS on cardiomyocyte damage and the expression of HIF-1α.Method：After the primary culture of neonatal rat cardiomyocytes were treated withby LPS,as indexes of cardiomyocyte damage,survival rate,LDH leakge rate,trypanblau uptake rate and CK activity were measured.The expression of HIF-1α was assessed using Western blot.Result：LPS can could increase LDH leakge rate,trypanblau uptake rate and CK activity;depress survival rate.At the same time,LPS can could change the expression of HIF-1α.Conclusion：LPS can could induce cardiomyocyte damage and enhance the expression of HIF-1α,and the significance of HIF-1α needs further research.

关 键 词：心肌抑制 脂多糖 缺氧诱导因子-1Α 

分 类 号：R331.3[医药卫生—人体生理学]