^(125)I标记bFGF注入豚鼠腹腔后耳蜗和全身各脏器放射自显影观察  

Autoradiography observation on cochlea and organs inguineapigs after intra-abdominal injection of bFGF

作　　者：翟所强[1] 陈泮藻[2] 郭维[1] 于宁[1] 孙建和[1] 胡吟燕[1] 

机构地区：[1]解放军总医院耳鼻咽喉头颈外科医院,北京100853 [2]解放军总医院基础医学研究所生化室

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第16期750-752,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家科技支撑计划(No:2006BAI02B6);国家自然科学基金(No:30772413)

摘　　要：目的:观察碱性成纤维细胞生长因子(bFGF)能否通过血迷路屏障,为临床合理用药提供实验依据。方法:豚鼠30只分为3组:125I-bFGF腹腔注入(125I-bFGF组)10只,125I腹腔注入(125I组)10只,生理盐水腹腔注入(生理盐水组)10只。2h后,取血、脑、双耳蜗、甲状腺、心脏、肝脏、肾脏称重,于r放射免疫分析仪中测定每个样品的每分钟记数率。双耳蜗标本切片做放射自显影检查。结果:125I组各脏器每分钟放射含量最高,耳蜗标本切片放射自显影可见颗粒显现;125I-bFGF组各脏器每分钟放射含量以血中最高,耳蜗及脑内含量最低,耳蜗标本切片放射自显影未见颗粒显现。结论:125I标记的bFGF腹腔注入后透过血迷路屏障有一定困难,因此,bFGF临床用药途径值得探讨。Objective：To observe whether bFGF could cross the blood-labyrinth barrier（BLB）after intra-abdominal injection and to establish an experimental basis for its clinical applications.Method：Thirty guinea pigs were divided into three groups.Animals in group 1were admistered125I-bFGF,while animals in group 2and 3were admistered125I and saline,respectively,via intra-abdominal injection.The both cochlea,blood,liver,brain,thyroid gland and kidney were collected and weighted.A radioimmunoassay analyzer was employed to measure counts per minute（CPM）of each sample,and autoradiography was performed on both cochlea.Result：The CPM value of organ samples in the125I group was higher than that in other groups,and radioactive grain was observed in cochlear samples of this group.In the 125I-bFGF group,blood demonstrated the highest CPM value,while cochlea and brain demonstrated the lowest CPM value,with no radioactive grain observed in cochlear samples.Conclusion：bFGF has some difficulties in getting across BLB,so the way of bFGF application in clinics need further study.

关 键 词：碱性成纤维细胞生长因子 耳蜗 血迷路屏障 放射自显影 

分 类 号：R764[医药卫生—耳鼻咽喉科]