家蚕多肽抑制因子对酯酶A4的ATP酶活性影响  

Effects of Polypeptide Inhibitory Needle (PIN) on Activity of Esterase A4 in Vitro

作　　者：徐世清[1] 郑必平 陈息林 司马杨虎 虞晓华 甲斐英则[2] 徐俊良[3] 

机构地区：[1]苏州大学蚕桑学院 [2]日本国立鸟取大学农学部 [3]浙江大学蚕学系

出　　处：《蚕业科学》1999年第1期16-21,共6页ACTA SERICOLOGICA SINICA

摘　　要：从产下后２ｄ的家蚕Ｃ１０８品种滞育性卵精制酯酶Ａ４和其多肽抑制因子ＰＩＮ，体外调查了ＰＩＮ对酯酶Ａ４的ＡＴＰ酶活性影响。酯酶Ａ４和ＰＩＮ５℃混合２５～１３ｄ，除去ＰＩＮ后５℃保护，ＡＴＰ酶活性峰都在１２～１４ｄ后出现；２５℃混合时，除去ＰＩＮ后，ＡＴＰ酶活性峰出现时间为混合时间再加１２～１４ｄ。利用ＭＡＬＤＩＭＳ方法，首次检测到纯化的酯酶Ａ４和合成ＰＩＮ的结合体。Esterase A4 (Ease A4) and its polypeptide inhibitory needle (PIN) were purified from diapause eggs of C108 Bombyx mori 2 days after oviposition.Effects of PIN on ATPase activities of Ease A4 was investigated in vitro .The Ease A4 was incubated with PIN for 2.5～13 days at 5℃ or 25℃.After removing of the PIN by centricon 10,the Ease A4 was chilled at 5℃.When the Ease A4 was incubated at 5℃,the ATPase active peaks appeared at 12～14 days after removing of the PIN.While at 25℃,the peaks appeared at 12～14 days plus the incubation duration.By using the method of matrix assisted laser desorption ionization mass spectrometry (MALDIMS),the complex of purified Ease A4 and synthesized PIN was found for the first time.

关 键 词：家蚕 滞育性卵 体外 酯酶A4 多肽抑制栓 

分 类 号：S882.3[农业科学—特种经济动物饲养]