家蚕病原白僵菌原生质体的再生回复研究  

Studies on the Regeneration and Reversion of Protoplasts From Beauveria bassiana, a Pathogenic Fungus to Bombyx mori L.

作　　者：时连根[1] 柯林田 费建明 

机构地区：[1]浙江农业大学蚕学系 [2]浙江省湖州市蚕桑推广总站

出　　处：《蚕业科学》1999年第1期36-40,共5页ACTA SERICOLOGICA SINICA

基　　金：浙江省自然科学基金

摘　　要：研究了家蚕病原白僵菌（Ｂｅａｕｖｅｒｉａｂａｓｉａｎａ）原生质体的性状、再生回复形式与条件。家蚕病原白僵菌原生质体的无核率为２６５％，有核率为７３５％，其中单核率为５３５％。原生质体再生回复的形式可观察到３种，在位相差显微镜下不能判别原生质体有无再生回复能力。综合考虑原生质体再生频率和菌落生长发育速度，家蚕病原白僵菌原生质体再生回复用培养基的渗透压稳定剂以０７ｍｏｌ／Ｌ葡萄糖较为适当。Regeneration and reversion of protoplasts from pathogenic fungus, Beauveria bassiana of silkworm, Bombyx mori L.were studied.Among the protoplasts,26.5% were without a nucleus,53.5% contained a single nucleus,and the other protoplasts contained two or more nuclei.Three morphologically distinct regeneration and reversion types of the potoplasts were observed,and the regenerating protoplasts could not be distinguished from nonregenerating protoplasts under a phase contrast microscope.Through the comprehensive survey of the colony formation and growth of the protoplasts,it is suggested that glucose of 0.7 mol/L as osmotic stabilizers for regeneration and reversion of the protoplasts is more suitable.

关 键 词：家蚕 白僵菌 原生质体 再生回复 

分 类 号：S884.49[农业科学—特种经济动物饲养]