高效液相色谱-示差折光检测法同时测定复方苯海拉明搽剂中樟脑和薄荷脑的含量  

Simultaneous Assay of Camphor and Mentholum in Compound Diphenhydramine Hydrochloride Liniment by HPLC with RID

作　　者：张云楚[1] 郭宇[2] 纪宇[1] 

机构地区：[1]江苏省药品检验所,南京210008 [2]中国药科大学,南京210009

出　　处：《药学与临床研究》2010年第5期501-502,共2页Pharmaceutical and Clinical Research

摘　　要：目的:建立高效液相色谱-示差折光检测法测定复方苯海拉明搽剂中樟脑和薄荷脑含量的方法。方法:采用示差折光检测器,色谱柱采用十八烷基键合硅胶色谱柱(4.6mm×250mm,5μm);流动相为甲醇-水(80︰20);柱温为30℃;流速为1.0mL.min-1。结果:樟脑和薄荷脑分别在0.10～1.00 mg.mL-1和0.10～1.00 mg.mL-1的浓度范围内有良好的线性关系,r分别为0.9997和0.9998,两者的平均回收率分别为99.9%(RSD=1.0%)和99.5%(RSD=0.7%)。结论:此法简便,快速,结果准确可靠,适用于同时测定复方苯海拉明搽剂中樟脑和薄荷脑的含量。Objective： To establish methods using HPLC-RID for simultaneous assay ot camphor and mentholum in compound diphenhydramine hydrochloride liniment. Methods： The detector was a differential refraction detector. The Waters Symmetry C18（250mm×4.6 mm, 5 μm）was used at 30℃. The mobile phase was composed of methanol and water（80：20）. The flow rate was 1.0mL·min^-1. Results： The linear range of camphor and mentholum were 0.10-1.00 mg·mL^-1 （r=0.9997） and 0.10-1.00 mg·mL^-1（r=0.9998）, respectively. The average recovery of camphor was 99.9%, RSD was 1.0%. The average recovery of mentholum was 99.5%, RSD was 0.7%. Conclusions： This method is efficient,accurate and suitable for the simultaneous assay of camphor and mentholum in compoud diphenhydramine hydrochloride liniment.

关 键 词：复方苯海拉明搽剂 高效液相色谱 示差折光检测器 樟脑 薄荷脑 

分 类 号：R927.2[医药卫生—药学]