蛋白激酶A与精子活力关系的探讨  

Investigation of the relationship of protein kinase A and sperm motility

作　　者：倪佳[1] 高晓勤[2] 丁贤胜[1] 杨丹[1] 郑立宏[1] 

机构地区：[1]贵阳医学院组织胚胎学教研室,贵州贵阳550004 [2]遵义医药高等专科学校,贵州遵义563002

出　　处：《检验医学》2010年第10期749-752,共4页Laboratory Medicine

基　　金：贵州省高层次人才特助项目[TZJF-2009-37];贵州省科技厅攻关项目[黔科合S字(2007)1030]

摘　　要：目的研究蛋白激酶A（PKA）对精子活力的影响。方法收集36例年龄20~40岁不育男性精液,根据伟力彩色精子质量检测系统检查结果分为3组：不明原因不育组（9例）、少精或畸形精子多组（15例）、泌尿生殖系统炎症组（12例）。以17名20~40岁正常生育男性精液作为对照组。用酶联免疫吸附试验（ELISA）测定精液PKA含量;并采用伟力彩色精子质量检测系统分别记录每例的精子活力。结果 PKA含量与精子活力呈正相关（r=0.935,P〈0.01）。不育各组中的精子PKA含量和精子活力分别与正常生育组比较,差异均有统计学意义（P〈0.01）。结论精子PKA含量与男性不育存在一定的相关性,精子PKA含量与精子活力呈明显正相关,精子PKA含量是评定男性生育能力的重要指标之一。Objective To study the influence of protein kinase A（PKA） on sperm motility.Methods The semens of 36 infertile men from 20 to 40 years old were collected and classified into 3 groups by the results of the WEILI color sperm analysis system： unknown cause infertile group（9 cases）,little or many abnormal sperm group（15 cases） and urogenital system inflammation group（12 cases）.Healthy fertile men′s semens in the same age were the control group（17 cases）.The contents of PKA in the semens were determined by enzyme-linked immunosorbent assay（ELISA）.Sperm motility of each group was recorded by WEILI color sperm analysis system.Results The content of PKA was positively correlated with sperm motility（r=0.935,P0.01）.There was significant difference between healthy fertile group and infertile groups（P0.01）.Conclusions There is a certain association between the content of PKA in semen and male infertility.There is a significant positive correlation between PKA content and sperm motility.Sperm PKA content is one of the most important indicators to assess male fertility.

关 键 词：蛋白激酶A 精子活力 不育 

分 类 号：Q555[生物学—生物化学]